Document Detail


Transformation of chloroplasts with the psaB gene encoding a polypeptide of the photosystem I reaction center.
MedLine Citation:
PMID:  1959594     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A chloroplast photosystem I reaction center mutation, ac-u-g-2.3, of Chlamydomonas reinhardtii has been complemented with a wild type psaB gene to restore photosynthetic competence. The mutation was mapped in the psaB coding sequence by chloroplast transformation using subcloned restriction fragments of psaB. The mutation was found to be a single base pair deletion resulting in a reading frame shift and premature termination of the polypeptide. Transformants were verified by insertion of a site-directed mutation which created a new restriction enzyme site. These transformations demonstrate the feasibility of insertion of site-directed mutations into the psaB gene in order to elucidate amino acid residues involved in photosystem I assembly and function.
Authors:
S E Bingham; R H Xu; A N Webber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  FEBS letters     Volume:  292     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-01-07     Completed Date:  1992-01-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  137-40     Citation Subset:  IM    
Affiliation:
Department of Botany, Arizona State University, Tempe 85287-1601.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/S62644;  S62648;  S62652;  S67655;  S67792;  S68009;  X58803;  X63567;  X63568;  X63569
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
Chlamydomonas / genetics*
Chloroplasts*
Molecular Sequence Data
Mutagenesis, Site-Directed
Photosynthetic Reaction Center Complex Proteins / genetics*
Photosystem I Protein Complex
Restriction Mapping
Transformation, Genetic*
Chemical
Reg. No./Substance:
0/Photosynthetic Reaction Center Complex Proteins; 0/Photosystem I Protein Complex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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