| Transformation of chenodeoxycholic acid by thermophilic Geobacillus stearothermophilus. | |
| | |
MedLine Citation:
|
PMID: 21838799 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We performed a series of experiments with Geobacillus stearothermophilus, a thermophile isolated from oil-contaminated soil in the Kuwaiti desert. The organism has a good potential for the transformation of a broad spectrum of organic molecules such as steroids, amino acids, and aromatic hydrocarbons. In the present study, we tested its potential for the transformation of a bile component, chenodeoxycholic acid (CDCA). Five transformed products, namely, cholic acid, methylcholate, methylchenodeoxycholate, 3α-hydroxy-7-oxo-5β-cholanic acid, and 7α-hydroxy-3-oxo-5β-cholanic acid, were the major transformation products catalyzed by G. stearothermophilus. Under aerobic conditions, no evidence of side chain degradation, ring cleavage, or dehydrogenation was found among the metabolites of CDCA. CDCA transformation by a thermophile is reported for the first time. |
| | |
Authors:
|
Mohammad Afzal; Sosamma Oommen; Samira Al-Awadi |
Related Documents
:
|
1151629 - Alcoholysis of medicinally active 5-aminodibenzo(a,d)cycloheptenes. 17456929 - Use of fumaric acid esters in psoriasis. 15936739 - Analysis of acidic carbohydrates as their quaternary ammonium or phosphonium salts by m... 5903089 - Repair of thermal injury of staphylococcus aureus. 17753149 - Acid of krypton and its barium salt. 9419859 - Effects of salt and serum on the sporicidal activity of liquid disinfectants. 2263169 - Effects of lorglumide on gastrin- and peptone-stimulated gastric acid secretion in rats. 12514249 - Chloroplast transformation with modified accd operon increases acetyl-coa carboxylase a... 14273639 - Pyruvate metabolism, carbon dioxide assimilation, and nitrogen fixation by an achromoba... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-07-27 |
Journal Detail:
|
Title: Biotechnology and applied biochemistry Volume: 58 ISSN: 1470-8744 ISO Abbreviation: Biotechnol. Appl. Biochem. Publication Date: 2011 Jul-Aug |
Date Detail:
|
Created Date: 2011-08-15 Completed Date: 2011-12-07 Revised Date: 2012-01-25 |
Medline Journal Info:
|
Nlm Unique ID: 8609465 Medline TA: Biotechnol Appl Biochem Country: England |
Other Details:
|
Languages: eng Pagination: 250-5 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 International Union of Biochemistry and Molecular Biology, Inc. |
Affiliation:
|
Department of Biological Sciences, Kuwait University, Kuwait City. afzalq8@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biotransformation Chenodeoxycholic Acid / chemistry, metabolism* Geobacillus stearothermophilus / isolation & purification, metabolism* Hot Temperature Kuwait Soil Microbiology* |
| Chemical | |
Reg. No./Substance:
|
474-25-9/Chenodeoxycholic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Stable and high-level production of recombinant Factor IX in human hepatic cell line.
Next Document: Label-free electrochemical aptasensor for thrombin detection with platinum nanoparticles and blockin...