| Transformation of animal cells with human T-cell leukemia virus type II. | |
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MedLine Citation:
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PMID: 2118885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human T-cell leukemia virus type II (HTLV-II) was tested for its ability to transform normal animal cells. The HTLV-II-infected human T-cell line, HTLV-IIA was lethally X-irradiated and cocultivated with normal leukocytes of rabbit and crab-eating monkey and spleen cells of hamster. The transformed cell lines, designated Ra-IIA, Si-IIA and Ham-IIA, were established. These cell lines were shown to be infected with HTLV-II by the polymerase chain reaction method combined with the digoxigenin-enzyme-linked immunosorbent assay method. These cell lines were examined for viral antigens by the indirect immunofluorescence method. Although the cytoplasma of over 90% of the cells of Si-IIA cell line was brilliantly stained, Ra-IIA and Ham-IIA cells were not stained. Electron microscopy of cells of the Si-IIA line revealed C-type virus particles in the extracellular spaces. |
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Authors:
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K Miyamoto; N Tomita; K Hayashi; T Akagi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Japanese journal of cancer research : Gann Volume: 81 ISSN: 0910-5050 ISO Abbreviation: Jpn. J. Cancer Res. Publication Date: 1990 Aug |
Date Detail:
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Created Date: 1990-10-18 Completed Date: 1990-10-18 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8509412 Medline TA: Jpn J Cancer Res Country: JAPAN |
Other Details:
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Languages: eng Pagination: 720-2 Citation Subset: IM; X |
Affiliation:
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School of Health Sciences, Okayama University. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Line, Transformed Cell Transformation, Viral* Cricetinae DNA, Viral / analysis Haplorhini Human T-lymphotropic virus 2 / genetics, pathogenicity* Humans Molecular Sequence Data Rabbits |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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