Document Detail


Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop.
MedLine Citation:
PMID:  21785988     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The ability to transform chloroplasts in multiple species is important for improving agricultural traits. Chloroplast transformation of alfalfa (Medicago sativa L.), a useful forage plant with high market value, was achieved using a vector carrying aadA and gfp genes being introduced into the chloroplasts of alfalfa via particle bombardment using leaves and calli as explants. Resistant somatic embryos were generated and developed into plantlets from explants. The transformation efficiency was 1.3% for callus explants and 2.7% for leaf explants. PCR and Southern blotting analyses revealed that the foreign genes were integrated into the transformed chloroplast genome. The occurrence of GFP was further confirmed by fluorescence microscopy. Expression of foreign genes in alfalfa chloroplasts is therefore possible, and provides a novel means for genetic improvement of agronomically important traits and production of value-added proteins.
Authors:
Zhengyi Wei; Yanzhi Liu; Chunjing Lin; Yunpeng Wang; Qin'an Cai; Yingshan Dong; Shaochen Xing
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-23
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Biotechnology Research Centre, Jilin Academy of Agricultural Sciences, 1363, Caiyu Street, Changchun, 130033, People's Republic of China, weizy932@yahoo.com.cn.
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