| Transformation and action of extracellular NAD+ in perfused rat and mouse livers. | |
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MedLine Citation:
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PMID: 19079292 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Transformation and possible metabolic effects of extracellular NAD+ were investigated in the livers of mice (Mus musculus; Swiss strain) and rats (Rattus novergicus; Holtzman and Wistar strains). METHODS: The livers were perfused in an open system using oxygen-saturated Krebs/Henseleit-bicarbonate buffer (pH 7.4) as the perfusion fluid. The transformation of NAD+ was monitored using high-performance liquid chromatography. RESULTS: In the mouse liver, the single-pass metabolism of 100 micromol/L NAD+ was almost complete; ADP-ribose and nicotinamide were the main products in the outflowing perfusate. In the livers of both Holtzman and Wistar rats, the main transformation products were ADP-ribose, uric acid and nicotinamide; significant amounts of inosine and AMP were also identified. On a weight basis, the transformation of NAD+ was more efficient in the mouse liver. In the rat liver, 100 micromol/L NAD+ transiently inhibited gluconeogenesis and oxygen uptake. Inhibition was followed by a transient stimulation. Inhibition was more pronounced in the Wistar strain and stimulation was more pronounced in the Holtzman strain. In the mouse liver, no clear effects on gluconeogenesis and oxygen uptake were found even at 500 micromol/L NAD+. CONCLUSION: It can be concluded that the functions of extracellular NAD+ are species-dependent and that observations in one species are strictly valid for that species. Interspecies extrapolations should thus be made very carefully. Actually, even variants of the same species can demonstrate considerably different responses. |
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Authors:
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Ana Carla Broetto-Biazon; Fabrício Bracht; Livia Bracht; Ana Maria Kelmer-Bracht; Adelar Bracht |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-15 |
Journal Detail:
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Title: Acta pharmacologica Sinica Volume: 30 ISSN: 1745-7254 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-06 Completed Date: 2009-04-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: China |
Other Details:
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Languages: eng Pagination: 90-7 Citation Subset: IM |
Affiliation:
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Laboratory of Liver Metabolism, University of Maringá, 87020900 Maringá, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate Ribose
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metabolism Adenosine Monophosphate / metabolism Animals Chromatography, High Pressure Liquid / methods Gluconeogenesis / physiology Inosine / metabolism Liver / metabolism* Male Mice NAD / metabolism* Niacinamide / metabolism Oxygen Consumption Perfusion / methods Rats Rats, Sprague-Dawley / metabolism Rats, Wistar / metabolism Uric Acid / metabolism |
| Chemical | |
Reg. No./Substance:
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20762-30-5/Adenosine Diphosphate Ribose; 53-84-9/NAD; 58-63-9/Inosine; 61-19-8/Adenosine Monophosphate; 69-93-2/Uric Acid; 98-92-0/Niacinamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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