Document Detail


Transformation of NIH3T3 cells by Rous sarcoma virus occurs with high efficiency in the absence of proviral rearrangements or amplification.
MedLine Citation:
PMID:  1328985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NIH3T3 cells could be transformed by a mammaltropic strain of Rous sarcoma virus (RSV) with an efficiency 10(3) times greater than that observed in Balb/c 3T3 cells or other mammalian cell lines and almost identical to that of chick embryo fibroblasts. In infected NIH3T3 cells a single, properly integrated, provirus was sufficient to induce focus formation; moreover, kinase activity of pp60v-src and tyrosine phosphorylation of cellular proteins could be detected very soon after infection in the majority of cells. On the other hand, in transformed foci from RSV-infected Balb/c 3T3 cells both rearrangements and amplification of proviral sequences were frequently detected. Accordingly, expression of pp60v-src and ensuing tyrosine phosphorylation of cellular proteins occurred, at high levels, only in a minority of the infected cells. Furthermore, by using a murine retrovirus carrying the v-src oncogene and an independent selectable marker, we found that Balb/c 3T3 cells were transformed with a 100-fold lower efficiency than NIH3T3 cells, yet the majority of infected untransformed Balb/c 3T3 cells expressed active pp60v-src. These findings are consistent with the existence in most mammalian cell lines of a major restriction to v-src-induced transformation, operating at the level of proviral expression, that is apparently absent in NIH3T3 cells.
Authors:
G Falcone; C Provenzano; S Alemà; F Tatò
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  7     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-10-26     Completed Date:  1992-10-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1913-20     Citation Subset:  IM    
Affiliation:
Istituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Avian Sarcoma Viruses / genetics*
Cell Transformation, Neoplastic*
Cell Transformation, Viral*
Gene Amplification*
Gene Rearrangement*
Genes, src*
Mice
Oncogene Protein pp60(v-src) / analysis
Proviruses / genetics*
Chemical
Reg. No./Substance:
EC 2.7.10.2/Oncogene Protein pp60(v-src)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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