Document Detail


Transformation of Metarhizium anisopliae mediated by Agrobacterium tumefaciens.
MedLine Citation:
PMID:  16917517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple, highly efficient, and reliable Agrobacterium tumefaciens-mediated transformation method was developed for the insect pathogenic fungus Metarhizium anisopliae. Expression of the green fluorescent protein gene, egfp, and the benomyl resistance gene, benA3, were used as markers in transformed M. anisopliae. Transformation efficiencies were dependent on the strain of A. tumefaciens used. With strain AGL-1, 17.0 +/- 1.4 transformants per plate could be obtained using conidial concentrations of 10(6) conidia/mL and a 2 day co-cultivation in the presence of 200 micromol/L acetosyringone. On the other hand, transformations using strain LBA4404 were unsuccessful. Ten transformants were tested by Southern analysis and found to contain a single copy T-DNA. Twenty transformants were subcultured for five generations on nonselective media, and 95% of the transformants were mitotically stable. Agrobacterium tumefaciens-mediated transformation of M. anisopliae can serve as a useful tool to investigate genes involved in insect pathogenicity.
Authors:
Weiguo Fang; Yan Pei; Michael J Bidochka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  52     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-08-18     Completed Date:  2006-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  623-6     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Brock University, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascomycota / genetics*,  pathogenicity
Genes, Reporter / genetics
Genetic Markers
Genetic Vectors / biosynthesis*
Green Fluorescent Proteins / genetics
Insects / microbiology
Rhizobium radiobacter / genetics*
Transformation, Genetic*
Chemical
Reg. No./Substance:
0/Genetic Markers; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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