Document Detail


Transformation of Escherichia coli in foodstuffs.
MedLine Citation:
PMID:  10390866     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The plasmid transfer by transformation of Escherichia coli in 12 foods was investigated under conditions commonly found in processing and storage of food. Transformation occurred in all foods with frequencies of at least 10(-8) when a simplified standard transformation protocol with non-growing cells was applied. Higher rates (ca. 10(-7)) were found in milk, soy drink, tomato and orange juice. Furthermore, E. coli became transformed at temperatures below 5 degrees C, i.e. under conditions highly relevant in storage of perishable foods. In soy drink this condition resulted in frequencies which were even higher than those determined after application of a temperature shift to 37 degrees C. The transformation of cells growing in milk and carrot juice at a constantly kept temperature of 37 degrees C provides evidence for the potential of E. coli to become transformed naturally. With purified DNA frequencies were determined in these substrates of ca. 2.5 x 10(-7) and 2.5 x 10(-8), respectively. Similar frequencies were also obtained in milk containing the crude nucleic acids of homogenised cell suspensions of E. coli (pUC18). Moreover, the release of plasmid DNA from E. coli during food processing and the subsequent uptake of this DNA by growing E. coli cells was shown to take place after homogenisation in milk indicating a horizontal plasmid transfer by transformation of E. coli.
Authors:
F Bauer; C Hertel; W P Hammes
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Systematic and applied microbiology     Volume:  22     ISSN:  0723-2020     ISO Abbreviation:  Syst. Appl. Microbiol.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-07-22     Completed Date:  1999-07-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8306133     Medline TA:  Syst Appl Microbiol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  161-8     Citation Subset:  IM    
Affiliation:
Institut für Lebensmitteltechnologie, Universität Hohenheim, Stuttgart, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Beverages / microbiology
Culture Media / chemistry
Escherichia coli / genetics*,  growth & development
Food Handling
Milk / microbiology
Plasmids / genetics
Soybean Proteins
Temperature
Time Factors
Transfection / genetics*
Transformation, Bacterial / genetics*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Soybean Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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