Document Detail


Transformation of Bacillus polymyxa with plasmid DNA.
MedLine Citation:
PMID:  2604393     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A plasmid transformation system was developed for Bacillus polymyxa ATCC 12321 and derivatives of this strain. The method utilizes a penicillin-treated-cell technique to facilitate uptake of the plasmid DNA. Low-frequency transformation (10(-6) per recipient cell) of plasmids pC194, pBD64, and pBC16 was accomplished with this method. Selection for the transformants was accomplished on both hypertonic and nonhypertonic selective media, with the highest rates of recovery occurring on a peptone-glucose-yeast extract medium containing 0.25 M sucrose. Several additional plasmids were shown to be capable of transferring their antibiotic resistance phenotypes to B. polymyxa through the use of a protoplast transformation procedure which allowed for a more efficient transfer of the plasmid DNA. However, cell walls could not be regenerated on the transformed protoplasts, and the transformants could not be subcultured from the original selective media.
Authors:
D H Mallonee; R A Speckman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  55     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1990-02-02     Completed Date:  1990-02-02     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2517-21     Citation Subset:  IM    
Affiliation:
Department of Food Science, University of Illinois, Urbana 61801.
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MeSH Terms
Descriptor/Qualifier:
Bacillus / drug effects,  genetics*
Electrophoresis, Agar Gel
Penicillin Resistance / genetics
Penicillins / pharmacology
Phenotype
Plasmids*
Protoplasts / metabolism
Transformation, Bacterial*
Chemical
Reg. No./Substance:
0/Penicillins
Comments/Corrections

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