Document Detail

Transformation of Bacillus polymyxa with plasmid DNA.
MedLine Citation:
PMID:  2604393     Owner:  NLM     Status:  MEDLINE    
A plasmid transformation system was developed for Bacillus polymyxa ATCC 12321 and derivatives of this strain. The method utilizes a penicillin-treated-cell technique to facilitate uptake of the plasmid DNA. Low-frequency transformation (10(-6) per recipient cell) of plasmids pC194, pBD64, and pBC16 was accomplished with this method. Selection for the transformants was accomplished on both hypertonic and nonhypertonic selective media, with the highest rates of recovery occurring on a peptone-glucose-yeast extract medium containing 0.25 M sucrose. Several additional plasmids were shown to be capable of transferring their antibiotic resistance phenotypes to B. polymyxa through the use of a protoplast transformation procedure which allowed for a more efficient transfer of the plasmid DNA. However, cell walls could not be regenerated on the transformed protoplasts, and the transformants could not be subcultured from the original selective media.
D H Mallonee; R A Speckman
Related Documents :
17481973 - Separation of topological forms of plasmid dna by anion-exchange hplc: shifts in elutio...
16917953 - Alkaline-cell lysis through in-line static mixer reactor for the production of plasmid ...
18755053 - Dna topoisomerases: harnessing and constraining energy to govern chromosome topology.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  55     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1990-02-02     Completed Date:  1990-02-02     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2517-21     Citation Subset:  IM    
Department of Food Science, University of Illinois, Urbana 61801.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacillus / drug effects,  genetics*
Electrophoresis, Agar Gel
Penicillin Resistance / genetics
Penicillins / pharmacology
Protoplasts / metabolism
Transformation, Bacterial*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Evidence for a new pathway in the bacterial degradation of 4-fluorobenzoate.
Next Document:  Reductive dehalogenation of dichloroanilines by anaerobic microorganisms in fresh and dichlorophenol...