Document Detail


Transformation of 4-cholesten-3-one and 7 alpha-hydroxy-4-cholesten-3-one into cholestanol and bile acids in cerebrotendinous xanthomatosis.
MedLine Citation:
PMID:  6735073     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to determine whether cholestanol and bile acids are derived from the same precursor, key intermediates of both biosynthetic pathways beyond cholesterol were administered intravenously to a patient with cerebrotendinous xanthomatosis and to a control subject. After pulse-labeling with [4-14C]4-cholesten-3-one and [G-3H]7 alpha-hydroxy-4-cholesten-3-one, cholestanol, cholesterol, and the two primary bile acids, cholic acid and chenodeoxycholic acid were isolated from specimens of bile. Each compound was purified by thin-layer chromatography and conclusively identified by gas-liquid chromatography-mass spectrometry. In other studies, the in vitro formation of 4-cholesten-3-one from cholesterol was measured in hepatic microsomal fractions prepared from a subject with cerebrotendinous xanthomatosis and from 3 control individuals. In all subjects, cholic acid and chenodeoxycholic acid were labeled with tritium, but neither cholesterol nor cholestanol contained this isotope. In contrast, 14C was detected in the cholestanol fraction with trace amounts in chenodeoxycholic acid, cholic acid, and cholesterol. Hepatic microsomes prepared from liver biopsy specimens obtained from a subject with cerebrotendinous xanthomatosis produced three times more 4-cholesten-3-one than the controls. The results indicate that 4-cholesten-3-one was converted primarily into cholestanol and 7 alpha-hydroxy-4-cholesten-3-one into cholic acid and chenodeoxycholic acid. Neither ketonic steroid was transformed into cholesterol. The increased production of cholestanol in cerebrotendinous xanthomatosis may be accounted for by enhanced hepatic formation of 4-cholesten-3-one. 7 alpha-Hydroxy-4-cholesten-3-one is a precursor of bile acids, but not of cholestanol.
Authors:
G Salen; S Shefer; G S Tint
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gastroenterology     Volume:  87     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  1984 Aug 
Date Detail:
Created Date:  1984-08-14     Completed Date:  1984-08-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  276-83     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Bile Acids and Salts / biosynthesis*
Brain Diseases / metabolism*
Carbon Radioisotopes / diagnostic use
Chenodeoxycholic Acid / biosynthesis
Cholestanols / biosynthesis*
Cholestenes / metabolism*
Cholestenones / metabolism*
Cholesterol / metabolism
Cholic Acid
Cholic Acids / biosynthesis
Humans
Male
Microsomes, Liver / analysis
Middle Aged
Tendons*
Xanthomatosis / metabolism*
Grant Support
ID/Acronym/Agency:
AM 18707/AM/NIADDK NIH HHS; AM 26756/AM/NIADDK NIH HHS; HL 17818/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Carbon Radioisotopes; 0/Cholestanols; 0/Cholestenes; 0/Cholestenones; 0/Cholic Acids; 3862-25-7/7 alpha-hydroxy-4-cholesten-3-one; 474-25-9/Chenodeoxycholic Acid; 57-88-5/Cholesterol; 601-57-0/cholest-4-en-3-one; 81-25-4/Cholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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