Document Detail

Transferrin receptors and selective iron deposition in pancreatic B cells of iron-overloaded rats.
MedLine Citation:
PMID:  1776464     Owner:  NLM     Status:  MEDLINE    
Iron overload was produced in Wistar rats by repeated intraperitoneal injections of ferric nitrilotriacetate (Fe(3+)-NTA) for one to six months. Pancreatic tissues from these iron-overloaded rats and untreated controls were examined for insulin (for B cells), glucagon (for A cells), transferrin receptor (TfR), transferrin (Tf) and ferritin (Ft) using immunohistochemical methods, and for iron by histochemical Berlin blue staining. In the islets of iron-overloaded rats, increased Ft staining appeared prior to deposition of Berlin blue-stainable iron, and the staining intensity of Ft and iron was stronger in B cells than in A cells. In the islets of untreated control rats, the staining intensity of TfR was stronger in B cells than in A cells. TfR staining of the islets was weaker in iron-overloaded rats than in the controls. These findings suggest that 1) iron uptake by islet cells in vivo is regulated and mediated by TfR, 2) intracytoplasmic Ft transforms into stainable iron in iron-overloaded rats, and 3) predominance of TfR expression in B cells may result in selective deposition of iron and predispose B cells to damage and diabetes mellitus in iron-overloaded rats.
J P Lu; K Hayashi; S Okada; M Awai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pathologica japonica     Volume:  41     ISSN:  0001-6632     ISO Abbreviation:  Acta Pathol. Jpn.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1992-03-03     Completed Date:  1992-03-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372637     Medline TA:  Acta Pathol Jpn     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  647-52     Citation Subset:  IM    
Department of Pathology, Okayama University Medical School, Japan.
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MeSH Terms
Coloring Agents
Ferritins / metabolism
Immunoenzyme Techniques
Iron / poisoning*
Islets of Langerhans / drug effects*,  metabolism
Rats, Inbred Strains
Receptors, Transferrin / physiology*
Transferrin / metabolism
Reg. No./Substance:
0/Coloring Agents; 0/Ferrocyanides; 0/Receptors, Transferrin; 11096-37-0/Transferrin; 14038-43-8/ferric ferrocyanide; 7439-89-6/Iron; 9007-73-2/Ferritins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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