Document Detail

Transferrin and ferritin endocytosis and recycling in guinea-pig reticulocytes.
MedLine Citation:
PMID:  3036246     Owner:  NLM     Status:  MEDLINE    
Transferrin and ferritin endocytosis and exocytosis by guinea-pig reticulocytes were studied using incubation with pronase at 4 degrees C to distinguish internalized and membrane-bound protein. Internalization of both transferrin and ferritin occurred in a time- and temperature-dependent fashion. Transferrin endocytosis was more rapid than that of ferritin. Transferrin binding to receptors was not altered, but transferrin endocytosis was decreased in the presence of ferritin. Iron accumulation from transferrin was inhibited by ferritin to a greater extent than could be accounted for by the decreased rate of endocytosis. In pulse-chase experiments, almost all of the transferrin was released intact from reticulocytes, but only about 50% of the total internalized ferritin was released, of which 85% was intact. The endocytosis of transferrin by rabbit reticulocytes was 2- to 2.5-times faster than guinea-pig reticulocytes. These data suggest that ferritin and transferrin are internalized by receptor-mediated endocytosis, possibly involving the same coated pits and vesicles, but that the proteins are recycled only partly in common.
G D Blight; E H Morgan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  929     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-07-28     Completed Date:  1987-07-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  18-24     Citation Subset:  IM    
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MeSH Terms
Biological Transport / drug effects
Coated Pits, Cell-Membrane / metabolism
Ferritins / metabolism*,  pharmacology
Guinea Pigs
Iron / metabolism
Iron-Binding Proteins*
Receptors, Cell Surface / metabolism
Receptors, Transferrin / metabolism
Reticulocytes / metabolism*
Transferrin / metabolism*
Reg. No./Substance:
0/Iron-Binding Proteins; 0/Receptors, Cell Surface; 0/Receptors, Transferrin; 0/ferritin receptor; 11096-37-0/Transferrin; 7439-89-6/Iron; 9007-73-2/Ferritins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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