| Transferrin is an essential factor for myelination. | |
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MedLine Citation:
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PMID: 9972870 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been established that oligodendrocytes, the myelin forming cells, participate in iron homeostasis through the synthesis and secretion of transferrin. Here we investigated whether a correlation exists between myelination, the commonly studied function of oligodendrocytes, and that of transferrin synthesis and secretion. We used a proteolipid protein mutant, the myelin deficient rat, whose condition is characterized by severe hypomyelination. We compared the ontogenic profile for transferrin gene expression in mutants with that of unaffected rat pups through northern blot analysis and in situ hybridization. Surprisingly, transferrin synthesis was null in mutant oligodendrocytes. Next, we demonstrated that a single apo-transferrin intraparenchymal injection administered to P5 rat pups enabled mutant oligodendrocytes to synthesize myelin basic protein and to myelinate axons, indicating that transferrin effects mutant oligodendrocyte maturation regardless of its source. Thus, transferrin availability is essential for oligodendrocyte maturation and function, and oligodendrocytes are most vulnerable to transferrin deficiency during the premyelinating stage. |
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Authors:
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A Espinosa de los Monteros; S Kumar; P Zhao; C J Huang; R Nazarian; T Pan; S Scully; R Chang; J de Vellis |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neurochemical research Volume: 24 ISSN: 0364-3190 ISO Abbreviation: Neurochem. Res. Publication Date: 1999 Feb |
Date Detail:
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Created Date: 1999-03-30 Completed Date: 1999-03-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7613461 Medline TA: Neurochem Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 235-48 Citation Subset: IM |
Affiliation:
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Mental Retardation Research Center, Department of Neurobiology, Brain Research Institute, UCLA School of Medicine, Los Angeles, CA 90024-1759, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism Fluorescent Antibody Technique Gene Expression Regulation, Developmental In Situ Hybridization Myelin Basic Proteins / biosynthesis*, genetics, metabolism RNA, Messenger / genetics, metabolism Rats Rats, Mutant Strains Transferrin / genetics, metabolism, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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HD-04612/HD/NICHD NIH HHS; HD-06576/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Myelin Basic Proteins; 0/RNA, Messenger; 11096-37-0/Transferrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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