Document Detail


Transferrin is an essential factor for myelination.
MedLine Citation:
PMID:  9972870     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been established that oligodendrocytes, the myelin forming cells, participate in iron homeostasis through the synthesis and secretion of transferrin. Here we investigated whether a correlation exists between myelination, the commonly studied function of oligodendrocytes, and that of transferrin synthesis and secretion. We used a proteolipid protein mutant, the myelin deficient rat, whose condition is characterized by severe hypomyelination. We compared the ontogenic profile for transferrin gene expression in mutants with that of unaffected rat pups through northern blot analysis and in situ hybridization. Surprisingly, transferrin synthesis was null in mutant oligodendrocytes. Next, we demonstrated that a single apo-transferrin intraparenchymal injection administered to P5 rat pups enabled mutant oligodendrocytes to synthesize myelin basic protein and to myelinate axons, indicating that transferrin effects mutant oligodendrocyte maturation regardless of its source. Thus, transferrin availability is essential for oligodendrocyte maturation and function, and oligodendrocytes are most vulnerable to transferrin deficiency during the premyelinating stage.
Authors:
A Espinosa de los Monteros; S Kumar; P Zhao; C J Huang; R Nazarian; T Pan; S Scully; R Chang; J de Vellis
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  24     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-30     Completed Date:  1999-03-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  235-48     Citation Subset:  IM    
Affiliation:
Mental Retardation Research Center, Department of Neurobiology, Brain Research Institute, UCLA School of Medicine, Los Angeles, CA 90024-1759, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism
Fluorescent Antibody Technique
Gene Expression Regulation, Developmental
In Situ Hybridization
Myelin Basic Proteins / biosynthesis*,  genetics,  metabolism
RNA, Messenger / genetics,  metabolism
Rats
Rats, Mutant Strains
Transferrin / genetics,  metabolism,  physiology*
Grant Support
ID/Acronym/Agency:
HD-04612/HD/NICHD NIH HHS; HD-06576/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Myelin Basic Proteins; 0/RNA, Messenger; 11096-37-0/Transferrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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