Document Detail


Transfer of steroidal and nonsteroidal compounds across guinea pig fetal membranes.
MedLine Citation:
PMID:  9542156     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transfer of steroidal and nonsteroidal compounds across guinea pig amnion and chorion laeve was investigated as a function of stage of gestation, tissue orientation, steroid specificity, and molecular size. Each fetal membrane was examined at early and late stages of gestation, before and after pubic symphysis relaxation. Early amnion was impermeable to macromolecules and small charged molecules while [3H]estrone and [3H]pregnenolone were transferred, the latter depending on tissue orientation and involving conjugation at the basolateral interface. After symphysis dilation, amnion transferred all substrates tested with the exception of BSA; the molecular weight cutoff was approximately 5,000. Unlike amnion, early chorion transferred both free and conjugated steroids as well as inorganic sulfate. Transfer of estrone involved conjugation and depended on tissue orientation. Transfer of [3H]estrone-sulfate, [3H]estrone-glucuronide, and [3H]pregnenolone-sulfate was similar despite selective deconjugating activity toward estrone-sulfate. Near term, chorion was impermeable to inorganic sulfate and transfer of estrone-glucuronide depended on tissue orientation, involving deconjugation in the maternal to fetal direction. At no stage of gestation did chorion transfer macromolecules. These results suggest that the transfer of free and conjugated steroids across fetal membranes is differentially regulated by tissue, its stage of development, and direction of transfer.
Authors:
D E Goldhawk; R Hobkirk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  280     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-08     Completed Date:  1998-05-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  403-12     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Western Ontario, London, Canada. goldhawk@julian.uwo.ca
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MeSH Terms
Descriptor/Qualifier:
Amnion / metabolism
Animals
Chorion / metabolism
Estrogens, Conjugated (USP) / metabolism*
Estrone / analogs & derivatives,  metabolism
Extraembryonic Membranes / metabolism*
Female
Gestational Age
Guinea Pigs
Pregnancy
Pregnenolone / metabolism
Steroids / metabolism*
Chemical
Reg. No./Substance:
0/Estrogens, Conjugated (USP); 0/Steroids; 1247-64-9/pregnenolone sulfate; 145-13-1/Pregnenolone; 2479-90-5/estrone-3-glucuronide; 481-97-0/estrone sulfate; 53-16-7/Estrone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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