Document Detail


Transfer of a plasmid determining bacteriocin Bc-48 production and immunity, and response to sexual pheromones in Enterococcus faecalis S-48.
MedLine Citation:
PMID:  1518913     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Production of bacteriocin Bc-48 by Enterococcus faecalis S-48 is encoded by the conjugative plasmid pMB1, which is approximately 90 kb and also responds to sex pheromones of E. faecalis OG1X. Mutants harboring deleted forms of this plasmid (pMB1-del, 75 kb) have lost both the phenotype Bc-48 (production and immunity) and the clumping response. The conjugal transfer of pMB1 to E. faecalis OG1X results in the acquisition by this strain of both bacteriocin production and immunity and also the clumping response. In the transconjugants isolated, the bacteriocinogenic trait is associated with a smaller plasmid (52 kb), which we call pMB1-1. The relationship among plasmids pMB1, pMB1-del, and pMB1-1 has been demonstrated by DNA hybridization. Plasmid pMB1-1 has been transferred with high frequency to E. faecalis mutants cured of Bc-48 production (carrying pMB1-del), conferring to them the Bc-48 trait and clumping response. In the transconjugants from a second mating, pMB1-1 and pMB1-del coexist without appreciable segregation.
Authors:
M Martínez-Bueno; E Valdivia; A Gálvez; M Maqueda
Related Documents :
3828983 - Active h-ras and n-ras in rat fibrosarcomas induced by 1,6-dinitropyrene.
342513 - Expression of the uvrb gene of escherichia coli: in vitro construction of a pmb9 uvrb p...
18658133 - Protein-nanocrystal conjugates support a single filament polymerization model in r1 pla...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plasmid     Volume:  28     ISSN:  0147-619X     ISO Abbreviation:  Plasmid     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-10-07     Completed Date:  1992-10-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7802221     Medline TA:  Plasmid     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  61-9     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics*,  pharmacology
Bacteriocins / biosynthesis*,  pharmacology
Conjugation, Genetic
Crosses, Genetic
Drug Resistance, Microbial
Enterococcus faecalis / drug effects,  genetics*
Genetic Variation
Phenotype
Pheromones / pharmacology*
Plasmids / genetics*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacteriocins; 0/Pheromones; 0/bacteriocin Bc 48

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Selection of Dictyostelium discoideum transformants and analysis of vector maintenance using live ba...
Next Document:  Plasmid pTB913 derivatives are segregationally stable in Bacillus subtilis at elevated temperatures.