Document Detail


Transfer of macromolecules into living adult cardiomyocytes by microinjection.
MedLine Citation:
PMID:  9278237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Among techniques commonly used to deliver bioactive molecules into living cells, microinjection is a very efficient method. Microinjection has been used extensively for gene transfer into different cell types. We applied the microinjection technique to the adult rat ventricular cardiac muscle cells (AVC) in primary culture and optimized microinjection parameters and the appropriate cell culture conditions. We also optimized the use of particular agents (i.e. 2,3-butanedione monoxime, verapamil) for the prevention of the cell damage caused by the micropuncture. We obtained the expression of a CMV-beta-galactosidase reporter gene in up to 20% of the injected cells with efficient maintenance of long term cell viability. Under our experimental conditions direct microinjection is a very advantageous technique to transfer macromolecules into living adult cardiac muscle cells and a powerful system to study and manipulate the biochemistry and molecular biology of the cardiac myocyte.
Authors:
M Bartoli; W C Claycomb
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  172     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-10-14     Completed Date:  1997-10-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  103-9     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans 70112, USA.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Cell Culture Techniques / methods
Cells, Cultured
Female
Heart Ventricles / cytology
Macromolecular Substances
Microinjections / instrumentation,  methods*
Myocardium / cytology*
Rats
Chemical
Reg. No./Substance:
0/Macromolecular Substances

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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