Document Detail

Transfer of allergic airway responses with antigen-primed CD4+ but not CD8+ T cells in brown Norway rats.
MedLine Citation:
PMID:  7657805     Owner:  NLM     Status:  MEDLINE    
Activated CD4+ helper T cells have been demonstrated in asthmatic airways and postulated to play a central role in eliciting allergic inflammation; direct evidence of their involvement seems to be lacking. We hypothesized that CD4+ T cells have the potential to induce allergic responses to antigen challenge, and tested this hypothesis in a model of allergic bronchoconstriction, the Brown Norway rat, using the approach of adoptive transfer. Animals were actively sensitized to either ovalbumin (OVA) or BSA and were used as donors of T cells. W3/25(CD4)+ or OX8(CD8)+ T cells were isolated from the cervical lymph nodes of sensitized donors and transferred to naive BN rats. 2 d after adoptive transfer recipient rats were challenged by OVA inhalation, and changes in lung resistance (RL), bronchoalveolar lavage (BAL) cells, and serum levels of antigen-specific IgE were studied. After OVA challenge recipients of OVA-primed W3/25+ T cells exhibited sustained increases in RL throughout the entire 8-h observation period and had significant bronchoalveolar lavage eosinophilia, which was detected by immunocytochemistry using an antimajor basic protein mAb. Recipients of BSA-primed W3/25+ T cells or OVA-primed OX8+ T cells failed to respond to inhaled OVA. OVA-specific immunoglobulin E was undetectable by ELISA or skin testing in any of the recipient rats after adoptive transfer. In conclusion, antigen-induced airway bronchoconstriction and eosinophilia were successfully transferred by antigen-specific W3/25+ T cells in Brown Norway rats. These responses were dependent on antigen-primed W3/25+ T cells and appeared to be independent of IgE-mediated mast cell activation. This study provides clear evidence for T cell mediated immune mechanisms in allergic airway responses in this experimental model.
A Watanabe; H Mishima; P M Renzi; L J Xu; Q Hamid; J G Martin
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  96     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-10-04     Completed Date:  1995-10-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1303-10     Citation Subset:  AIM; IM; X    
Meakins-Christie Laboratories, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
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MeSH Terms
Allergens / immunology*
Bronchoalveolar Lavage Fluid / cytology
CD4-Positive T-Lymphocytes / immunology*
CD8-Positive T-Lymphocytes / immunology
Eosinophils / cytology,  immunology
Hypersensitivity / immunology*
Immunity, Cellular
Immunoglobulin E / analysis
Immunotherapy, Adoptive*
Leukocyte Count
Lung / immunology*
Lymph Nodes / immunology
Lymphocyte Activation
Lymphocyte Transfusion
Ovalbumin / immunology
Rats, Inbred BN
Respiratory System / immunology*
Serum Albumin, Bovine / immunology
Skin Tests
T-Lymphocyte Subsets / immunology*
Reg. No./Substance:
0/Allergens; 0/Serum Albumin, Bovine; 37341-29-0/Immunoglobulin E; 9006-59-1/Ovalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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