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Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates.
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MedLine Citation:
PMID:  6096826     Owner:  NLM     Status:  MEDLINE    
The primary sequences of all the tRNA precursors which contain intervening sequences and which accumulate in the Saccharomyces cerevisiae rnal mutant are presented. A combination of DNA and RNA sequence analysis has led to elucidation of the primary sequence of four hitherto uncharacterized precursors. The location of the intervening sequence has in all cases been unambiguously determined by analysis of the intermediates in the splicing reaction. Secondary structures based upon the tRNA cloverleaf are shown for all the tRNA precursors and discussed with respect to common recognition by the yeast splicing endonuclease.
R C Ogden; M C Lee; G Knapp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  12     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-02-21     Completed Date:  1985-02-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  9367-82     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M12370;  X01656;  X01657;  X01658;  X01659;  X01660
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MeSH Terms
Base Sequence
Cloning, Molecular
DNA Restriction Enzymes / diagnostic use
Nucleic Acid Conformation
Nucleic Acid Precursors / metabolism
RNA Splicing*
RNA, Fungal / genetics*
RNA, Transfer / metabolism*
Saccharomyces cerevisiae / genetics*
Grant Support
Reg. No./Substance:
0/Nucleic Acid Precursors; 0/RNA, Fungal; 9014-25-9/RNA, Transfer; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 21 Month: 12 Year: 1984
Volume: 12 Issue: 24
First Page: 9367 Last Page: 9382
ID: 320467
PubMed Id: 6096826

Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates.
R C Ogden
M C Lee
G Knapp

Article Categories:
  • Research Article

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