Document Detail

Transfection of murine P19S18 embryonal carcinoma cells with the oncogene neu induces an epithelioid phenotype.
MedLine Citation:
PMID:  1361918     Owner:  NLM     Status:  MEDLINE    
An embryonal carcinoma cell line, P19S18, was transfected with the rat oncogene neu to investigate the function of its protein product, p185*, in a multipotential cellular environment. Levels of message for p185* were determined by in situ hybridization analysis and two highly expressing clones, PnnA and PnnB, were isolated. As demonstrated by indirect immunofluorescence and immunoprecipitation, these neu-transfected cells synthesized a full length rat p185*. The transfectants do not resemble typical embryonal carcinoma cells either before or after differentiation is induced by retinoic acid treatment. They are much larger, flatter, "epithelioid" cells. These cells have lost the expression of stage specific embryonic antigen-1 (SSEA-1), but do synthesize and assemble the basement membrane components laminin and fibronectin. These results suggest that expression of the neu oncogene in a multipotential cell line may induce the synthesis of proteins indicative of an epithelioid phenotype due to the presence of p185*.
M C Kibbey; J E Mazurkiewicz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  51     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1993-02-03     Completed Date:  1993-02-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  129-35     Citation Subset:  IM    
Department of Anatomy, Cell Biology, and Neurobiology, Albany Medical College, NY 12208.
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MeSH Terms
Cell Differentiation / genetics
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation
In Situ Hybridization
Neoplasm Transplantation
Neoplasms, Germ Cell and Embryonal / pathology*
Proto-Oncogene Proteins / physiology*
Receptor, erbB-2
Tumor Cells, Cultured
Tumor Markers, Biological / physiology*
Reg. No./Substance:
0/Proto-Oncogene Proteins; 0/Tumor Markers, Biological; EC, erbB-2

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