| Transfection-mediated cell synchronization: acceleration of G1-S phase transition by gamma irradiation. | |
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MedLine Citation:
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PMID: 11730009 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously provided evidence that the uptake of DNA into cells is cell cycle specific following transfection. We show here that, immediately after transfection, successfully transfected cells are greatly enriched for cells in early G1 or G0 phase and that, upon removal of the DNA precipitates, cells progress through G1 and enter S phase in a synchronous fashion. We also demonstrate that this approach can be utilized in meaningful cell-cycle experiments, and we show that gamma irradiation accelerates the G1-S phase transition in a cell line with a functionally inactive p53 protein. |
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Authors:
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E J Jung; E K Flemington |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: BioTechniques Volume: 31 ISSN: 0736-6205 ISO Abbreviation: BioTechniques Publication Date: 2001 Nov |
Date Detail:
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Created Date: 2001-12-03 Completed Date: 2002-04-18 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 8306785 Medline TA: Biotechniques Country: United States |
Other Details:
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Languages: eng Pagination: 1026, 1028, 1031-4 Citation Subset: IM |
Affiliation:
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Tulane Health Sciences Center, New Orleans, LA 70112, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle Proteins DNA-Binding Proteins G1 Phase / radiation effects* Gamma Rays Genetic Vectors Hela Cells Humans Protein-Serine-Threonine Kinases / physiology S Phase / radiation effects* Transfection* Tumor Suppressor Proteins |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM48045/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Tumor Suppressor Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/ataxia telangiectasia mutated protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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