Document Detail


Transfection-mediated cell synchronization: acceleration of G1-S phase transition by gamma irradiation.
MedLine Citation:
PMID:  11730009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously provided evidence that the uptake of DNA into cells is cell cycle specific following transfection. We show here that, immediately after transfection, successfully transfected cells are greatly enriched for cells in early G1 or G0 phase and that, upon removal of the DNA precipitates, cells progress through G1 and enter S phase in a synchronous fashion. We also demonstrate that this approach can be utilized in meaningful cell-cycle experiments, and we show that gamma irradiation accelerates the G1-S phase transition in a cell line with a functionally inactive p53 protein.
Authors:
E J Jung; E K Flemington
Related Documents :
8299729 - Different fate of sibling cells upon inhibition of transcription in g1.
8166459 - Elutriation of t-lymphoblastic cells and cell cycle analysis of fractions.
7196799 - In vivo cell cycle phase-preferential killing of murine fibrosarcoma cells by cisplatin.
1270529 - The cell cycle during amphibian limb regeneration.
12573009 - Cell growth arrest by nucleotides, nucleosides and bases as a tool for improved product...
199259 - Synthesis and secretion of albumin by a synchronized rat hepatoma cell line.
2427339 - Biological studies of ten human squamous carcinoma cell lines: an overview.
2983489 - Immunohistochemical distribution of s-100 protein and glial fibrillary acidic protein i...
21154769 - Involvement of vilip-1 (visinin-like protein) and opposite roles of cyclic amp and gmp ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  BioTechniques     Volume:  31     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-12-03     Completed Date:  2002-04-18     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1026, 1028, 1031-4     Citation Subset:  IM    
Affiliation:
Tulane Health Sciences Center, New Orleans, LA 70112, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Cycle Proteins
DNA-Binding Proteins
G1 Phase / radiation effects*
Gamma Rays
Genetic Vectors
Hela Cells
Humans
Protein-Serine-Threonine Kinases / physiology
S Phase / radiation effects*
Transfection*
Tumor Suppressor Proteins
Grant Support
ID/Acronym/Agency:
R01 GM48045/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Tumor Suppressor Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/ataxia telangiectasia mutated protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES...
Next Document:  Cre stoplight: a red/green fluorescent reporter of Cre recombinase expression in living cells.