Document Detail

Transfection-mediated cell synchronization: acceleration of G1-S phase transition by gamma irradiation.
MedLine Citation:
PMID:  11730009     Owner:  NLM     Status:  MEDLINE    
We have previously provided evidence that the uptake of DNA into cells is cell cycle specific following transfection. We show here that, immediately after transfection, successfully transfected cells are greatly enriched for cells in early G1 or G0 phase and that, upon removal of the DNA precipitates, cells progress through G1 and enter S phase in a synchronous fashion. We also demonstrate that this approach can be utilized in meaningful cell-cycle experiments, and we show that gamma irradiation accelerates the G1-S phase transition in a cell line with a functionally inactive p53 protein.
E J Jung; E K Flemington
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  BioTechniques     Volume:  31     ISSN:  0736-6205     ISO Abbreviation:  BioTechniques     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-12-03     Completed Date:  2002-04-18     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  8306785     Medline TA:  Biotechniques     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1026, 1028, 1031-4     Citation Subset:  IM    
Tulane Health Sciences Center, New Orleans, LA 70112, USA.
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MeSH Terms
Cell Cycle Proteins
DNA-Binding Proteins
G1 Phase / radiation effects*
Gamma Rays
Genetic Vectors
Hela Cells
Protein-Serine-Threonine Kinases / physiology
S Phase / radiation effects*
Tumor Suppressor Proteins
Grant Support
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Tumor Suppressor Proteins; EC Kinases; EC telangiectasia mutated protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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