Document Detail


Transfection and ligation of CD40 in human oral keratinocytes affect proliferation, adhesion and migration but not apoptosis in vitro.
MedLine Citation:
PMID:  16487107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: CD40 expression is restricted to Keratinocytes of normal epidermis or stratified squamous epithelium of oral mucosa. Ligation of CD40 inhibits keratinocyte proliferation and apoptosis. The aim of this study was to investigate the functional significance of CD40 in the proliferation, apoptosis, adhesion and migration of human oral keratinocytes in vitro. METHODS: The CD40-negative oral keratinocyte line OSC19, its CD40-positive transfected derivative (OSC19T-CD40) and null transfectants (OSC19T-control), with and without stimulation by soluble protein CD40 ligand (sCD40L) or anti-CD40 antibodies were used. RESULTS: OSC19T-CD40 showed significantly (P < 0.001) slower growth than the null transfectants and parent cells. OSC19T-CD40 proliferation was inhibited by ligation with sCD40L and blocking by two anti-CD40 antibodies, but stimulated by a third. Binding of CD40 with ligand or antibody had no effect on keratinocyte apoptosis in any cell line. The capacity of OSC19T-CD40 cells to adhere to CD40L-coated wells was significantly greater (P < 0.001) than that of parent OSC19 and OSC19T-control cells, and the migration rate of OSC19T-CD40 cells was significantly higher than parent OSC19 (P = 0.038 on fibronectin, P = 0.004 on Matrigel) or OSC19T-control (P =0.017 on fibronectin, P = 0.013 on Matrigel) cells. CONCLUSIONS: CD40 is an important molecule in keratinocyte homeostasis, and has more than one ligand. The ligand that is bound may be critical in oral epithelial homeostasis, the development of malignancy and the behaviour of the subsequent tumour.
Authors:
M Villarroel Dorrego; S A Whawell; P M Speight; A W Barrett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental dermatology     Volume:  31     ISSN:  0307-6938     ISO Abbreviation:  Clin. Exp. Dermatol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7606847     Medline TA:  Clin Exp Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  266-71     Citation Subset:  IM    
Affiliation:
Oral and Maxillofacial Pathology Unit, Eastman Dental Institute for Oral Healthcare Sciences, University College London, London, UK.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD40 / biosynthesis,  pharmacology*
Apoptosis / drug effects
CD40 Ligand / metabolism
Cell Adhesion
Cell Movement
Cell Proliferation / drug effects
Epidermis / drug effects,  metabolism
Epithelium / drug effects,  metabolism
Humans
Keratinocytes / drug effects*,  metabolism*
Mouth Mucosa / drug effects*,  metabolism*
Chemical
Reg. No./Substance:
0/Antigens, CD40; 147205-72-9/CD40 Ligand

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