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Transfection of insect cell lines using polyethylenimine.
MedLine Citation:
PMID:  19002899     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Insect cell lines have been widely used in recombinant baculovirus expression systems and transient gene expression studies. Critical to these applications have been the transfection of foreign DNA. This has been frequently done using labor intensive and cytotoxic liposome-based transfection reagents. In the current study we have optimized a new kind of polyethylenimine-based DNA transfection reagent on the Spodoptera frugiperda Sf9 insect cell line. A plasmid vector that transiently expresses green fluorescent protein (GFP) was effectively delivered into Sf9 cells. A transfection efficiency of 54% and cell viability of 85-90% were obtained for Sf9 cells. The developed transfection protocol has now been successfully used to transfect eight insect cell lines derived from Bombyx mori, Trichoplusia ni, Helicoverpa zea, Heliothis virescens and S. frugiperda with GFP and GUS with transfection efficiencies of at least 45%. This method provides high heterologous protein expression levels, transfection efficacy and cell viability, and could be used for transient gene expression in other lepidopteran cell lines.
Authors:
I D Ogay; O A Lihoradova; Sh S Azimova; A A Abdukarimov; J M Slack; D E Lynn
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Publication Detail:
Type:  Journal Article     Date:  2006-11-02
Journal Detail:
Title:  Cytotechnology     Volume:  51     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2008-11-12     Completed Date:  2011-07-14     Revised Date:  2013-03-13    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  89-98     Citation Subset:  -    
Affiliation:
Institute of Genetics and Plant Experimental Biology, Uzbek Academy of Science Tashkent region, Qibray district, Yuqori-Yuz, 702151, Uzbekistan.
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