Document Detail


Transfection-induced tumor necrosis factor-alpha increases the susceptibility of human glioma cells to lysis by lymphokine-activated killer cells: continuous expression of intercellular adhesion molecule-1 on the glioma cells.
MedLine Citation:
PMID:  7915264     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To develop more effective adoptive immunotherapy, we transfected the human tumor necrosis factor-alpha (TNF-alpha) gene into human glioma cells (U251-SP), which were used as target cells. TNF-alpha is known to increase both the expression of intercellular adhesion molecule-1 (ICAM-1) on the surface of glioma cells and the susceptibility of glioma cells to lymphokine-activated killer (LAK) cell cytolysis. We compared the expression of ICAM-1 induced by TNF-alpha generated by the TNF-alpha gene-transfected cells with that induced by exogenously added TNF-alpha. When the TNF-alpha gene was transfected into U251-SP cells, the expression of ICAM-1 was detected on the cell surface from 3 days after the transfection and continued until at least 9 days. In contrast, it was expressed only transiently in the case of exogenously added TNF-alpha. Also, the cytolytic activity of LAK cells induced by transfection-induced TNF-alpha was significantly stronger than that induced by exogenously added TNF-alpha. The increased susceptibility was quenched by anti-ICAM-1 monoclonal antibody. These data indicated that continuous expression of ICAM-1 induced by TNF-alpha gene transfection of glioma cells resulted in higher cytolytic activity of LAK cells.
Authors:
T Takaoka; J Yoshida; M Mizuno; K Sugita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of cancer research : Gann     Volume:  85     ISSN:  0910-5050     ISO Abbreviation:  Jpn. J. Cancer Res.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-09-29     Completed Date:  1994-09-29     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  8509412     Medline TA:  Jpn J Cancer Res     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  750-5     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Nagoya University School of Medicine.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / pharmacology
Cell Adhesion Molecules / biosynthesis*,  immunology,  physiology
Flow Cytometry
Glioma / immunology*
Humans
Intercellular Adhesion Molecule-1
Killer Cells, Lymphokine-Activated / immunology*
Liposomes
Plasmids
Transfection*
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / genetics*,  pharmacology,  physiology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Cell Adhesion Molecules; 0/Liposomes; 0/Tumor Necrosis Factor-alpha; 126547-89-5/Intercellular Adhesion Molecule-1

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