| Transfection in Agrobacterium tumefaciens. | |
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MedLine Citation:
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PMID: 5040385 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intact cells of Agrobacterium tumefaciens were examined for ability to take up biologically active LR-4 phage deoxyribonucleic acid (DNA) from the surrounding medium. DNA incorporation as measured by subsequent plaque formation (transfection) failed to occur when the bacteria were grown in defined minimal salts media, and was restricted to a 4-hr period in the early log phase of growth in enriched media. In the latter case, maximal transfection frequencies were obtained after a 25- to 30-min incubation with 22.5 mug of phage DNA/ml. Higher DNA concentrations or longer incubation times were inhibitory. Transfection was completely inhibited by deoxyribonuclease but not by ribonuclease, trypsin, or phage-specific antisera. |
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Authors:
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V J Milani; G T Heberlein |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of virology Volume: 10 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1972 Jul |
Date Detail:
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Created Date: 1972-09-05 Completed Date: 1972-09-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 17-22 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriophages* Culture Media DNA, Viral* / isolation & purification Deoxyribonucleases / pharmacology Immune Sera / pharmacology Micropore Filters Rhizobium* / growth & development Ribonucleases / pharmacology Temperature Time Factors Transformation, Genetic Trypsin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/DNA, Viral; 0/Immune Sera; EC 3.1.-/Deoxyribonucleases; EC 3.1.-/Ribonucleases; EC 3.4.21.4/Trypsin |
| Comments/Corrections | |
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