| Transfection by electroporation. | |
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MedLine Citation:
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PMID: 18428436 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Electroporation--the use of high-voltage electric shocks to introduce DNA into cells--can be used with most cell types,yields a high frequency of both stable transformation and transient gene expression,and,because it requires fewer steps,can be easier than alternate techniques. In this unit,the basic protocol describes the electroporation of mammalian cells and an alternate protocol outlines modifications for preparation and transfection of plant protoplasts. |
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Authors:
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H Potter |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.] Volume: Appendix 1 ISSN: 1934-8576 ISO Abbreviation: Curr Protoc Neurosci Publication Date: 2001 May |
Date Detail:
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Created Date: 2008-04-22 Completed Date: 2008-05-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706581 Medline TA: Curr Protoc Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: Appendix 1E Citation Subset: IM |
Affiliation:
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Harvard Medical School, Boston, Massachusetts, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA / administration & dosage Electroporation / methods* Mammals Transfection / methods* |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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