| Transepithelial transport of fluorescein in Caco-2 cell monolayers and use of such transport in in vitro evaluation of phenolic acid availability. | |
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MedLine Citation:
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PMID: 12506986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescein is a marker-dye customary applied to the evaluation of tight-junctional permeability of epithelial cell monolayers. However, the true mechanism for the permeation has not been elucidated. Transepithelial transport of fluorescein in Caco-2 cell monolayers was therefore examined. Fluorescein transport was dependent on pH, and in a vectorical way in the apical-basolateral direction, but it was independent of the tight-junctional permeability of monolayers of these human intestinal cells. The permeation of fluorescein was concentration-dependent and saturable; the Michaelis constant was 7.7 mM and the maximum velocity was 40.3 nmol min(-1) (mg protein)(-1). Benzoic acid competitively inhibited fluorescein transport, suggesting that fluorescein is transported by a monocarboxylic acid transporter (MCT). Antioxidative polyphenolic compounds such as ferulic acid from dietary sources, competitively inhibited the permeation of fluorescein. These compounds probably share a transport carrier with fluorescein. Measurement of the effects of phenolic acids on fluorescein transport across Caco-2 monolayers would be a useful way to evaluate the intestinal absorption or bioavailability of dietary phenolic acids. |
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Authors:
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Yutaka Konishi; Keiko Hagiwara; Makoto Shimizu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 66 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-12-31 Completed Date: 2003-07-07 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 2449-57 Citation Subset: IM |
Affiliation:
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Applied Bioresearch Center, Research & Development Dept., Kirin Brewery Co., Ltd., 3 Miyaharacho, Takasaki-shi, Gunma 370-1295, Japan. konishiy@kirin.co.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Benzoic Acid
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pharmacology Biological Availability Caco-2 Cells Coumaric Acids / pharmacology Drug Interactions Electric Impedance Epithelial Cells / metabolism Fluorescein / pharmacokinetics* Humans Hydrogen-Ion Concentration Hydroxybenzoic Acids / pharmacokinetics* Intestinal Absorption / drug effects Intestinal Mucosa / cytology, metabolism* Isoquinolines / metabolism Monocarboxylic Acid Transporters / metabolism* Tight Junctions / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
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0/Coumaric Acids; 0/Hydroxybenzoic Acids; 0/Isoquinolines; 0/Monocarboxylic Acid Transporters; 1135-24-6/ferulic acid; 2321-07-5/Fluorescein; 29656-58-4/phenolic acid; 65-85-0/Benzoic Acid; 77944-88-8/lucifer yellow |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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