| Transepithelial transport of bepridil in the human intestinal cell line, Caco-2, using a "dynamic model". | |
| | |
MedLine Citation:
|
PMID: 12877575 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The purpose of the study was to go further into the transepithetial transport of bepridil, an anticalcic agent, through monolayer cells Caco-2, using a "dynamic model" including a transfer of inserts with Caco-2 cells into new wells, free of drug, at regular intervals, in order to simulate the blood flux. The state of cells was evaluated by measuring the transepithelial electrical resistance and the transport of bepridil was followed using a gas chromatography/mass spectrometry determination. This study exhibits the importance of the basolateral renewal both on the transport of bepridil and the maintenance of cells in a satisfactory state. Two elimination phases from the cell compartment seem to occur, with basolateral half lives respectively of 12.2 and 25.6 hours, probably linked with two kinds of cellular binding sites. This dynamic model permits the reflection and simulation of the slowness of the in vivo absorption of bepridil in the small intestine. |
| | |
Authors:
|
F Mathieu; M J Galmier; A Nicolay; J F Pognat; C Lartigue |
Related Documents
:
|
22705965 - Harvesting nannochloris oculata by inorganic electrolyte flocculation: effect of initia... 18044965 - Myristoyl-based transport of peptides into living cells. 8627565 - Transport characteristics of differently charged cephalosporin antibiotics in oocytes e... 803955 - Transport of d- and l-tryptophan in bacillus megaterium by an inducible permease. 7961875 - Gs-x pump is functionally overexpressed in cis-diamminedichloroplatinum (ii)-resistant ... 22705965 - Harvesting nannochloris oculata by inorganic electrolyte flocculation: effect of initia... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: European journal of drug metabolism and pharmacokinetics Volume: 28 ISSN: 0378-7966 ISO Abbreviation: Eur J Drug Metab Pharmacokinet Publication Date: 2003 Apr-Jun |
Date Detail:
|
Created Date: 2003-07-24 Completed Date: 2004-03-23 Revised Date: 2011-02-02 |
Medline Journal Info:
|
Nlm Unique ID: 7608491 Medline TA: Eur J Drug Metab Pharmacokinet Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 155-60 Citation Subset: IM |
Affiliation:
|
UMR INSERM 484, Laboratoire de Chimie Analytique et Spectrométrie de Masse, Faculté de Pharmacie, Clermont-Ferrand, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bepridil
/
pharmacokinetics* Biological Transport / physiology Caco-2 Cells Epithelial Cells / metabolism Humans Intestinal Mucosa / metabolism* Models, Biological* Models, Chemical* |
| Chemical | |
Reg. No./Substance:
|
64706-54-3/Bepridil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro approach to study the influence of the cardiac output distribution on drug concentration.
Next Document: Ionic modulation of the effects of heparin and 6-aminohexanoic acid on plasminogen activation by str...