Document Detail


Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers.
MedLine Citation:
PMID:  23265462     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of-flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6mM. The apparent permeability (P(app)) of CS in the apical-to-basolateral direction was 5.4×10(-7)cm/s, while in the reversed direction the P(app) value was significantly reduced (1.9×10(-7)cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway.
Authors:
Hoang Lan Phuong; Ju Qiu; Takashi Kuwahara; Keiichi Fukui; Kayo Yoshiyama; Kazusato Matsugano; Norihiko Terahara; Toshiro Matsui
Publication Detail:
Type:  Journal Article     Date:  2012-11-12
Journal Detail:
Title:  Food chemistry     Volume:  138     ISSN:  0308-8146     ISO Abbreviation:  Food Chem     Publication Date:  2013 May 
Date Detail:
Created Date:  2012-12-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7702639     Medline TA:  Food Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  101-6     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenviromental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
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