| Transepithelial transport of macromolecular substances in IL-4 treated human intestinal T84 cell monolayers. | |
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MedLine Citation:
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PMID: 19897912 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The effect of interleukin-4 (IL-4), a cytokine associated with allergy and inflammation, on the permeability of the intestinal epithelium was investigated. IL-4 reduced transepithelial electrical resistance (TER) and increased permeation to horseradish peroxidase (HRP) and Lucifer Yellow (LY) of human intestinal T84 cell monolayers. The increased permeation due to IL-4 treatment was also observed at 4 degrees C. The permeability of T84 cell monolayers to beta-lactogulobulin (beta-Lg), ovalbumin (OVA), and fluorescein isothiocyanate (FITC)-dextran of various molecular sizes was also high in the IL-4-treated cell monolayers. Sodium azide (NaN(3)), which inhibits ATP synthesis of the cells, did not inhibit the increases in these substances. Even 150 kDa FITC-dextran significantly permeated the T84 cells when the monolayers were treated with IL-4. These results suggest that fairly large molecules are able to permeate intestinal epithelial monolayers via the energy-independent paracellular pathway when the monolayers are exposed to excessive IL-4. |
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Authors:
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Tetsunosuke Mochizuki; Hideo Satsu; Mamoru Totsuka; Makoto Shimizu |
Publication Detail:
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Type: Journal Article Date: 2009-11-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 73 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 2422-6 Citation Subset: IM |
Affiliation:
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Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. atmochi@mail.ecc.u-tokyo.ac.jp |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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