Document Detail


Transepithelial transport of macromolecular substances in IL-4 treated human intestinal T84 cell monolayers.
MedLine Citation:
PMID:  19897912     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The effect of interleukin-4 (IL-4), a cytokine associated with allergy and inflammation, on the permeability of the intestinal epithelium was investigated. IL-4 reduced transepithelial electrical resistance (TER) and increased permeation to horseradish peroxidase (HRP) and Lucifer Yellow (LY) of human intestinal T84 cell monolayers. The increased permeation due to IL-4 treatment was also observed at 4 degrees C. The permeability of T84 cell monolayers to beta-lactogulobulin (beta-Lg), ovalbumin (OVA), and fluorescein isothiocyanate (FITC)-dextran of various molecular sizes was also high in the IL-4-treated cell monolayers. Sodium azide (NaN(3)), which inhibits ATP synthesis of the cells, did not inhibit the increases in these substances. Even 150 kDa FITC-dextran significantly permeated the T84 cells when the monolayers were treated with IL-4. These results suggest that fairly large molecules are able to permeate intestinal epithelial monolayers via the energy-independent paracellular pathway when the monolayers are exposed to excessive IL-4.
Authors:
Tetsunosuke Mochizuki; Hideo Satsu; Mamoru Totsuka; Makoto Shimizu
Publication Detail:
Type:  Journal Article     Date:  2009-11-07
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  73     ISSN:  1347-6947     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2422-6     Citation Subset:  IM    
Affiliation:
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. atmochi@mail.ecc.u-tokyo.ac.jp
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