| Transepithelial Transport of 4-Chloro-2-Methylphenoxyacetic Acid (MCPA) across Human Intestinal Caco-2 Cell Monolayers. | |
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MedLine Citation:
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PMID: 22181038 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Mechanisms of transcellular transport of 4-chloro-2-methylphenoxyacetic acid (MCPA) across the small intestine were investigated using Caco-2 cells cultured on permeable membranes. The cell monolayers were incubated with MCPA, either from apical side at pH 6.0 or 7.4, or basolateral side at pH 7.4. The accumulation and apical-to-basolateral transport of MCPA was markedly stimulated by the acidic pH on the apical side (inwardly directed H(+) -gradient), dependent on metabolic energy and inhibited by co-incubation with acetic acid or benzoic acid. Without the H(+) -gradient, on the other hand, the basolateral-to-apical transport of MCPA (secretory transport) was higher than the apical-to-basolateral transport (absorptive transport), although the secretory transport of MCPA was markedly lower than the absorptive transport under the H(+) -gradient. Co-incubation of MCPA with probenecid from the basolateral side significantly inhibited the accumulation and transport of MCPA, whereas co-incubation with p-aminohippuric acid did not. These results suggest that the absorptive transport of MCPA is mediated by H(+) -linked monocarboxylic acid transporters expressed on the apical membranes, while secretory transport is mediated by a probenecid-sensitive transporter expressed on the basolateral membranes of Caco-2 cell monolayers. |
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Authors:
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Osamu Kimura; Kensuke Tsukagoshi; Moriaki Hayasaka; Tetsuya Endo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-19 |
Journal Detail:
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Title: Basic & clinical pharmacology & toxicology Volume: - ISSN: 1742-7843 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101208422 Medline TA: Basic Clin Pharmacol Toxicol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Basic & Clinical Pharmacology & Toxicology © 2011 Nordic Pharmacological Society. |
Affiliation:
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Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Hokkaido, Japan Hijirigaoka Hospital, Hokkaido, Japan Sapporo Higashi-Tokushyukai Hospital, Sapporo, Hokkaido, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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