| Transepithelial D-glucose and D-fructose transport across the American lobster, Homarus americanus, intestine. | |
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MedLine Citation:
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PMID: 21697425 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Transepithelial transport of dietary d-glucose and d-fructose was examined in the lobster Homarus americanus intestine using d-[(3)H]glucose and d-[(3)H]fructose. Lobster intestines were mounted in a perfusion chamber to determine transepithelial mucosal to serosal (MS) and serosal to mucosal (SM) transport mechanisms of glucose and fructose. Both MS glucose and fructose transport, as functions of luminal sugar concentration, increased in a hyperbolic manner, suggesting the presence of mucosal transport proteins. Phloridizin inhibited the MS flux of glucose, but not that of fructose, suggesting the presence of a sodium-dependent (SGLT1)-like glucose co-transporter. Immunohistochemical analysis, using a goat anti-rabbit GLUT5 polyclonal antibody, revealed the localization of a brush border GLUT5-like fructose transport protein. MS fructose transport was decreased in the presence of mucosal phloretin in warm spring/summer animals, but the same effect was not observed in cold autumn/winter animals, suggesting a seasonal regulation of sugar transporters. Mucosal phloretin had no effect on MS glucose transport. Both SM glucose and SM fructose transport were decreased in the presence of increasing concentrations of serosal phloretin, providing evidence for the presence of a shared serosal GLUT2 transport protein for the two sugars. The transport of d-glucose and d-fructose across lobster intestine is similar to sugar uptake in mammalian intestine, suggesting evolutionarily conserved absorption processes for these solutes. |
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Authors:
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Ijeoma E Obi; Kenneth M Sterling; Gregory A Ahearn |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of experimental biology Volume: 214 ISSN: 1477-9145 ISO Abbreviation: J. Exp. Biol. Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-06-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0243705 Medline TA: J Exp Biol Country: England |
Other Details:
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Languages: eng Pagination: 2337-44 Citation Subset: IM |
Affiliation:
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Department of Biology, University of North Florida, 1 UNF Drive, Jacksonville, FL 32224, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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