| Transduction of human antigen-presenting cells with integrase-defective lentiviral vector enables functional expansion of primed antigen-specific CD8(+) T cells. | |
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MedLine Citation:
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PMID: 20210625 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nonintegrating lentiviral vectors are being developed as a efficient and safe delivery system for both gene therapy and vaccine purposes. Several reports have demonstrated that a single immunization with integration-defective lentiviral vectors (IDLVs) delivering viral or tumor model antigens in mice was able to elicit broad and long-lasting specific immune responses in the absence of vector integration. At present, no evidence has been reported showing that IDLVs are able to expand preexisting immune responses in the human context. In the present study, we demonstrate that infection of human antigen-presenting cells (APCs), such as monocyte-derived dendritic cells (DCs) and macrophages with IDLVs expressing influenza matrix M1 protein resulted in effective induction of in vitro expansion of M1-primed CD8(+) T cells, as evaluated by both pentamer staining and cytokine production. This is the first demonstration that IDLVs represent an efficient delivery system for gene transfer and expression in human APCs, useful for immunotherapeutic applications. |
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Authors:
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Donatella R M Negri; Roberta Bona; Zuleika Michelini; Pasqualina Leone; Iole Macchia; Mary E Klotman; Mirella Salvatore; Andrea Cara |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Human gene therapy Volume: 21 ISSN: 1557-7422 ISO Abbreviation: Hum. Gene Ther. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-11 Completed Date: 2011-01-18 Revised Date: 2012-05-01 |
Medline Journal Info:
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Nlm Unique ID: 9008950 Medline TA: Hum Gene Ther Country: United States |
Other Details:
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Languages: eng Pagination: 1029-35 Citation Subset: IM |
Affiliation:
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Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, 00161 Rome, Italy. donatella.negri@iss.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigen-Presenting Cells
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immunology,
virology* CD8-Positive T-Lymphocytes / immunology, virology* Dendritic Cells / immunology Gene Therapy Genetic Vectors* Humans Immunization Integrases / genetics, immunology Lentivirus / genetics, immunology Macrophages / immunology Monocytes / cytology, immunology Transduction, Genetic Viral Proteins / immunology |
| Grant Support | |
ID/Acronym/Agency:
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1R21AI073926-01A2/AI/NIAID NIH HHS; R21 AI073926/AI/NIAID NIH HHS; R21 AI073926-01A2/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Viral Proteins; EC 2.7.7.-/Integrases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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