Document Detail

Transduction of bovine adrenal chromaffin cells using a recombinant adenovirus expressing GFP.
MedLine Citation:
PMID:  12535768     Owner:  NLM     Status:  MEDLINE    
Insertion of genetic material into bovine chromaffin cells employing various techniques have produced low to moderate transduction rates. Recent technology using adenoviral gene transfer has become one of the most powerful methods for introducing genes into mammalian cells. We examined whether a recombinant adenovirus could provide a convenient vector to transfer genes of interest for mechanistic studies on chromaffin cells. Our results show that 100% transduction of chromaffin cells was accomplished within 18 h with a recombinant adenovirus as revealed by the expression of green fluorescent protein (GFP) in chromaffin cells. Transduction was dependent on time and viral titer but independent of cell age and density in culture. There was no effect of the recombinant adenovirus on the secretory function [3H]-norepinephrine ([3H]-NE release) of the chromaffin cells. The results demonstrate that the recombinant adenovirus provides an effective process for the complete transfection of bovine chromaffin cells with a selected gene.
Xinying Li; Desinee A Drakulich; Peijin Zhang; Meilan Shen; Gregory A Weber; Tsuneya Ikezu; Terry D Hexum
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  122     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2003-01-21     Completed Date:  2003-03-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  91-6     Citation Subset:  IM    
Department of Pharmacology, University of Nebraska Medical Center, 986260 Nebraska Medical Center, Omaha, NE 68198-6260, USA.
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MeSH Terms
Adenoviridae / genetics*
Adrenal Medulla / metabolism
Cells, Cultured
Chromaffin Cells / metabolism*
Gene Expression Regulation*
Genetic Vectors
Green Fluorescent Proteins
Luminescent Proteins / metabolism*
Norepinephrine / secretion
Recombination, Genetic*
Transduction, Genetic / methods*
Transfection / methods
Reg. No./Substance:
0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins; 51-41-2/Norepinephrine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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