| Transducer of Cdc42-dependent actin assembly promotes epidermal growth factor-induced cell motility and invasiveness. | |
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MedLine Citation:
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PMID: 21062739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Toca-1 (transducer of Cdc42-dependent actin assembly) interacts with the Cdc42·N-WASP and Abi1·Rac·WAVE F-actin branching pathways that function in lamellipodia formation and cell motility. However, the potential role of Toca-1 in these processes has not been reported. Here, we show that epidermal growth factor (EGF) induces Toca-1 localization to lamellipodia, where it co-localizes with F-actin and Arp2/3 complex in A431 epidermoid carcinoma cells. EGF also induces tyrosine phosphorylation of Toca-1 and interactions with N-WASP and Abi1. Stable knockdown of Toca-1 expression by RNA interference has no effect on cell growth, EGF receptor expression, or internalization. However, Toca-1 knockdown cells display defects in EGF-induced filopodia and lamellipodial protrusions compared with control cells. Further analyses reveal a role for Toca-1 in localization of Arp2/3 and Abi1 to lamellipodia. Toca-1 knockdown cells also display a significant defect in EGF-induced motility and invasiveness. Taken together, these results implicate Toca-1 in coordinating actin assembly within filopodia and lamellipodia to promote EGF-induced cell migration and invasion. |
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Authors:
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Jinghui Hu; Alka Mukhopadhyay; Andrew W B Craig |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-09 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 286 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-17 Completed Date: 2011-02-24 Revised Date: 2012-01-23 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 2261-72 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and the Cancer Biology and Genetics Division, Queen's University Cancer Research Institute, Queen's University Kingston, Ontario K7L 3N6, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actin-Related Protein 2-3 Complex
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genetics,
metabolism Actins / genetics, metabolism* Adaptor Proteins, Signal Transducing / genetics, metabolism Carrier Proteins / genetics, metabolism* Cell Line Cell Movement / drug effects, physiology* Cytoskeletal Proteins / genetics, metabolism Epidermal Growth Factor / metabolism*, pharmacology Gene Knockdown Techniques Humans Protein Transport / drug effects, physiology Pseudopodia / genetics, metabolism Wiskott-Aldrich Syndrome Protein / genetics, metabolism Wiskott-Aldrich Syndrome Protein Family / genetics, metabolism cdc42 GTP-Binding Protein / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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//Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/ABI1 protein, human; 0/Actin-Related Protein 2-3 Complex; 0/Actins; 0/Adaptor Proteins, Signal Transducing; 0/Carrier Proteins; 0/Cytoskeletal Proteins; 0/FNBP1L protein, human; 0/WAS protein, human; 0/WASF1 protein, human; 0/Wiskott-Aldrich Syndrome Protein; 0/Wiskott-Aldrich Syndrome Protein Family; 62229-50-9/Epidermal Growth Factor; EC 3.6.5.2/cdc42 GTP-Binding Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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