| Transduced human copper chaperone for Cu,Zn-SOD (PEP-1-CCS) protects against neuronal cell death. | |
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MedLine Citation:
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PMID: 16404156 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reactive oxygen species (ROS) contribute to the development of various human diseases. Cu,Zn-superoxide dismutase (SOD) is one of the major means by which cells counteract the deleterious effects of ROS. SOD activity is dependent upon bound copper ions supplied by its partner metallochaperone protein, copper chaperone for SOD (CCS). In the present study, we investigated the protective effects of PEP-1-CCS against neuronal cell death and ischemic insults. When PEP-1-CCS was added to the culture medium of neuronal cells, it rapidly entered the cells and protected them against paraquat-induced cell death. Moreover, transduced PEP-1-CCS markedly increased endogenous SOD activity in the cells. Immunohistochemical analysis revealed that it prevented neuronal cell death in the hippocampus in response to transient forebrain ischemia. These results suggest that CCS is essential to activate SOD, and that transduction of PEP-1-CCS provides a potential strategy for therapeutic delivery in various human diseases including stroke related to SOD or ROS. |
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Authors:
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Soo Hyun Choi; Dae Won Kim; So Young Kim; Jae Jin An; Sun Hwa Lee; Hee Soon Choi; Eun Jung Sohn; Seok-Il Hwang; Moo Ho Won; Tae-Cheon Kang; Hyung Joo Kwon; Jung Hoon Kang; Sung-Woo Cho; Jinseu Park; Won Sik Eum; Soo Young Choi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecules and cells Volume: 20 ISSN: 1016-8478 ISO Abbreviation: Mol. Cells Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2006-01-11 Completed Date: 2006-03-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9610936 Medline TA: Mol Cells Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 401-8 Citation Subset: IM |
Affiliation:
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Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astrocytes / metabolism Brain Ischemia / chemically induced, metabolism, pathology Cell Death / drug effects Copper / metabolism* Gerbillinae Hippocampus / drug effects Humans Immunoenzyme Techniques Male Molecular Chaperones / therapeutic use* Neurons / drug effects*, metabolism Oxidative Stress / physiology Paraquat / toxicity Recombinant Fusion Proteins / metabolism, therapeutic use Transfection |
| Chemical | |
Reg. No./Substance:
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0/CCS protein, human; 0/Molecular Chaperones; 0/Recombinant Fusion Proteins; 4685-14-7/Paraquat; 7440-50-8/Copper |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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