Document Detail

Transdifferentiation of rat hepatic stellate cells results in leptin expression.
MedLine Citation:
PMID:  9514867     Owner:  NLM     Status:  MEDLINE    
Leptin is a peptide hormone that appears critical in regulating Fat metabolism. Recently, circulating leptin levels were reported higher in patients with alcoholic cirrhosis. In health, hepatic stellate cells store retinoids, but following liver injury they transdifferentiate into myofibroblast-like cells with loss of the retinoid stores. Leptin expression was demonstrated by detection of leptin mRNA by RT-PCR analysis and by immunohistochemistry viewed with confocal microscopy in transdifferentiated stellate cells after 14 days, or more, of culture. Leptin expression was not found in freshly isolated quiescent stellate cells. Leptin expression was not demonstrated in freshly isolated or cultured Kupffer cells. Treatment of activated stellate cells with either 1 microM retionic acid or 10 microM retinol acetate resulted in the inhibition of leptin mRNA expression. The observation that activated stellate cells in culture can express leptin has implications for understanding adipocyte biology in liver disease and treatment of malnutrition in cirrhotics.
J J Potter; L Womack; E Mezey; F A Anania
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  244     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-04-09     Completed Date:  1998-04-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  178-82     Citation Subset:  IM    
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2195, USA.
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MeSH Terms
Cell Differentiation / drug effects,  genetics
Cell Separation
Cells, Cultured
Liver / cytology*,  drug effects,  metabolism*
Obesity / metabolism,  pathology
Protein Biosynthesis*
Proteins / antagonists & inhibitors,  genetics,  isolation & purification
RNA, Messenger / biosynthesis
Rats, Sprague-Dawley
Tretinoin / pharmacology
Vitamin A / analogs & derivatives,  pharmacology
Grant Support
Reg. No./Substance:
0/Leptin; 0/Proteins; 0/RNA, Messenger; 11103-57-4/Vitamin A; 127-47-9/retinol acetate; 302-79-4/Tretinoin
Erratum In:
Biochem Biophys Res Commun 1998 Jul 20;248(2):441

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