Document Detail


Transdifferentiation of Clara cell 10-kDa protein secreting cells in experimental allergic rhinitis.
MedLine Citation:
PMID:  21294974     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The reasons for the down-regulated Clara cell 10-kDa protein (CC10) expression in allergic rhinitis (AR) are unclear and the airway remodeling in AR has received little attention. This study explores epithelium remodeling and the change of CC10 secreting cells in AR by using a murine model.
METHODS: AR murine models were established by ovalbumin sensitization and challenge. In some mice, dexamethasone was given before each challenge. Histological changes of nasal mucosa were examined by means of hematoxylin and eosin and periodic acid-Schiff staining. CC10 and trefoil factor family (TFF) 1 expression were evaluated by immunohistochemistry.
RESULTS: In AR mice, both in turbinate and in septal mucosa, total cell number and the number of basal cells did not change; however, the number of dome-shaped cells decreased and the number of ciliated and goblet cells increased in turbinate mucosa, and the number of ciliated cells decreased and the number of goblet cells increased in septal mucosa. In turbinate mucosa, the number of CC10(+) cells (mainly dome-shaped cells) decreased whereas the number of TFF1(+) cells (mainly ciliated cells) increased. In septal mucosa, the number of CC10(+) and TFF1(+) cells (mainly ciliated cells) decreased simultaneously. Intermediate phenotypic goblet cells could express CC10 and TFF1. CC10 and TFF1 could be localized in the same cells. Dexamethasone reversed the changes of epithelium significantly.
CONCLUSION: Allergen exposure leads to a possible transdifferentiation of CC10 secreting cells into TFF1 secreting cells and/or goblet cells in upper airways. Nasal turbinate and septal epithelium display different patterns of transdifferentiation.
Authors:
Yong-Hua Cui; Yuan-Yuan Wang; Zheng Liu
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Publication Detail:
Type:  Journal Article     Date:  2011-01-28
Journal Detail:
Title:  American journal of rhinology & allergy     Volume:  25     ISSN:  1945-8932     ISO Abbreviation:  Am J Rhinol Allergy     Publication Date:    2011 May-Jun
Date Detail:
Created Date:  2011-06-17     Completed Date:  2011-11-07     Revised Date:  2011-11-10    
Medline Journal Info:
Nlm Unique ID:  101490775     Medline TA:  Am J Rhinol Allergy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  145-51     Citation Subset:  IM    
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Airway Remodeling / drug effects
Animals
Cell Count
Cell Transdifferentiation / drug effects
Dexamethasone / administration & dosage
Disease Models, Animal
Gene Expression Regulation / drug effects
Humans
Immunization
Mice
Mice, Inbred C57BL
Nasal Mucosa / pathology
Ovalbumin / administration & dosage
Peptides / genetics,  metabolism
Respiratory Mucosa / drug effects,  metabolism*,  pathology
Rhinitis, Allergic, Perennial / chemically induced,  drug therapy,  genetics,  metabolism*,  pathology*
Uteroglobin / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Clara cell secretory protein; 0/Peptides; 0/Tff1 protein, mouse; 50-02-2/Dexamethasone; 9006-59-1/Ovalbumin; 9060-09-7/Uteroglobin

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