Document Detail

Transdermal insulin application system with dissolving microneedles.
MedLine Citation:
PMID:  23013202     Owner:  NLM     Status:  In-Data-Review    
Abstract Background: The aim of this report was to develop a dissolving microneedle (DM) application system, where 225-300 insulin-loaded DMs were formed on a chip. After the heat-sealed sheet is removed, the system covered with the press-through package layer is put on the skin. By pressing with the hand, insulin DMs were inserted into the skin. Materials and Methods: Factors affecting the penetration depth of DM were studied using applicator in vitro and in vivo experiments. The penetration depth was determined for rat and human skin. Two-layered DM array chips were prepared to obtain complete absorption of insulin and administered to the rat abdominal skin. Plasma glucose levels were measured for 6 h. By comparing the hypoglycemic effect with that obtained after subcutaneous injection, relative pharmacological availability (RPA) was determined. Results: The penetration depth increased from 21±3 μm to 63±2 μm in proportion to application speed to isolated rat skin, at 0.8-2.2 m/s. Human skin showed similar results in the penetration depth. The in vivo penetration depth was dependent on the force (0.5-2.5 N) and duration (1-10 min), as the secondary application force. The penetration depth was 211±3 μm with a duration of 3 min in the in vivo rat experiment. DM array chips having an insulin-loaded space of 181.2±4.2 and 209±3.9 μm were evaluated in the rat. RPA values of insulin from DMs were 98.1±0.8% and 98.1±3.1%, respectively. Conclusions: These results suggest the usefulness of the two-layered DM application system for the transdermal delivery of insulin.
Yukako Ito; Takuya Nakahigashi; Naoko Yoshimoto; Yuriko Ueda; Noriyuki Hamasaki; Kanji Takada
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diabetes technology & therapeutics     Volume:  14     ISSN:  1557-8593     ISO Abbreviation:  Diabetes Technol. Ther.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889084     Medline TA:  Diabetes Technol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  891-9     Citation Subset:  IM    
Department of Pharmacokinetics, Kyoto Pharmaceutical University , Yamashina-ku, Kyoto, Japan .
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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