| Transdentinal protective role of sodium ascorbate against the cytopathic effects of H2O2 released from bleaching agents. | |
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MedLine Citation:
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PMID: 20303050 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture. STUDY DESIGN: Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers. MDPC-23 odontoblastlike cells were seeded on pulp surface of the discs and the following groups were established: G1-No Treatment (control), G2-10% SA/6hs, G3-10%/CP6hs, G4-10%SA/6hs+10%CP/6hs, G5-16%CP/6hs, and G6-10%SA/6hs+16%CP/6hs. The cell viability was measured by the MTT assay. RESULTS: In groups where 16% CP was used, decreased cell viability was observed. Conversely, the application of 10% SA on the dentin discs, before the use of the CP, reduced the cytotoxic effects of these products on cells. CONCLUSIONS: The 16% CP cause a significant decrease in MDPC-23 cell viability and 10% SA was able to partially prevent the toxic effects of CP. |
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Authors:
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Adriano Fonseca Lima; Fernanda Campos Rosetti Lessa; Maria Nadir Gasparoto Mancini; Josimeri Hebling; Carlos Alberto de Souza Costa; Giselle Maria Marchi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics Volume: 109 ISSN: 1528-395X ISO Abbreviation: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-22 Completed Date: 2010-07-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508562 Medline TA: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Country: United States |
Other Details:
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Languages: eng Pagination: e70-6 Citation Subset: D; IM |
Copyright Information:
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Copyright 2010 Mosby, Inc. All rights reserved. |
Affiliation:
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Department of Restorative Dentistry, Piracicaba School of Dentistry, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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therapeutic use* Ascorbic Acid / therapeutic use* Cell Adhesion / drug effects Cell Culture Techniques Cell Line Cell Survival / drug effects Coloring Agents / diagnostic use Dentin / drug effects* Dentin Permeability / drug effects Diffusion Humans Hydrogen Peroxide / toxicity* Materials Testing Microscopy, Electron, Scanning Odontoblasts / drug effects Oxidants / toxicity* Peroxides / toxicity Protective Agents / therapeutic use* Spectrophotometry, Ultraviolet Tetrazolium Salts / diagnostic use Thiazoles / diagnostic use Time Factors Tooth Bleaching* Urea / analogs & derivatives, toxicity |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Coloring Agents; 0/Oxidants; 0/Peroxides; 0/Protective Agents; 0/Tetrazolium Salts; 0/Thiazoles; 124-43-6/carbamide peroxide; 298-93-1/thiazolyl blue; 50-81-7/Ascorbic Acid; 57-13-6/Urea; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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