Document Detail

Transdentinal protective role of sodium ascorbate against the cytopathic effects of H2O2 released from bleaching agents.
MedLine Citation:
PMID:  20303050     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture. STUDY DESIGN: Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers. MDPC-23 odontoblastlike cells were seeded on pulp surface of the discs and the following groups were established: G1-No Treatment (control), G2-10% SA/6hs, G3-10%/CP6hs, G4-10%SA/6hs+10%CP/6hs, G5-16%CP/6hs, and G6-10%SA/6hs+16%CP/6hs. The cell viability was measured by the MTT assay. RESULTS: In groups where 16% CP was used, decreased cell viability was observed. Conversely, the application of 10% SA on the dentin discs, before the use of the CP, reduced the cytotoxic effects of these products on cells. CONCLUSIONS: The 16% CP cause a significant decrease in MDPC-23 cell viability and 10% SA was able to partially prevent the toxic effects of CP.
Adriano Fonseca Lima; Fernanda Campos Rosetti Lessa; Maria Nadir Gasparoto Mancini; Josimeri Hebling; Carlos Alberto de Souza Costa; Giselle Maria Marchi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics     Volume:  109     ISSN:  1528-395X     ISO Abbreviation:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-22     Completed Date:  2010-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508562     Medline TA:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e70-6     Citation Subset:  D; IM    
Copyright Information:
Copyright 2010 Mosby, Inc. All rights reserved.
Department of Restorative Dentistry, Piracicaba School of Dentistry, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil.
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MeSH Terms
Antioxidants / therapeutic use*
Ascorbic Acid / therapeutic use*
Cell Adhesion / drug effects
Cell Culture Techniques
Cell Line
Cell Survival / drug effects
Coloring Agents / diagnostic use
Dentin / drug effects*
Dentin Permeability / drug effects
Hydrogen Peroxide / toxicity*
Materials Testing
Microscopy, Electron, Scanning
Odontoblasts / drug effects
Oxidants / toxicity*
Peroxides / toxicity
Protective Agents / therapeutic use*
Spectrophotometry, Ultraviolet
Tetrazolium Salts / diagnostic use
Thiazoles / diagnostic use
Time Factors
Tooth Bleaching*
Urea / analogs & derivatives,  toxicity
Reg. No./Substance:
0/Antioxidants; 0/Coloring Agents; 0/Oxidants; 0/Peroxides; 0/Protective Agents; 0/Tetrazolium Salts; 0/Thiazoles; 124-43-6/carbamide peroxide; 298-93-1/thiazolyl blue; 50-81-7/Ascorbic Acid; 57-13-6/Urea; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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