Document Detail


Transcutaneous determination of tissue dihematoporphyrin ether content. A device to optimize photodynamic therapy.
MedLine Citation:
PMID:  1845278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photodynamic therapy involves the use of light of appropriate wavelength to excite a photosensitizer resulting in tissue destruction. The photosensitizer dihematoporphyrin ether is selectively retained in tumors allowing for tumor destruction while sparing normal structures. Accessibility of skin tumors makes them well suited for photodynamic therapy. Tissue and tumor dihematoporphyrin ether content is estimated based on the amount of dihematoporphyrin ether administered. In our study, skin dihematoporphyrin ether content was measured in guinea pigs transcutaneously by a hand-held fluorometer and compared with dihematoporphyrin ether determinations done on skin biopsy specimens. Fluorometry was performed on guinea pigs receiving 0, 2.5, 5, 10, and 25 mg/kg of dihematoporphyrin ether. Transcutaneous measurements of skin fluorescence increased with increasing dihematoporphyrin ether dose and correlated well with skin dihematoporphyrin ether content as determined by extracting dihematoporphyrin ether from skin samples. Transcutaneous fluorescent measurements of guinea pigs given 0 and 2.5, 2.5 and 5, 5 and 10, and 10 and 25 mg/kg of dihematoporphyrin ether differed in a statistically significant manner. Transcutaneous fluorometric determination of dihematoporphyrin ether content and extraction of dihematoporphyrin ether from skin samples were able to reflect differences in dihematoporphyrin ether dosing and presumably skin dihematoporphyrin ether content. However, transcutaneous fluorometry provides an instantaneous estimate of tissue dihematoporphyrin ether without the need for a tissue sample. This may provide a clinical tool to predict more accurately the optimal light dose necessary to maximize photodynamic therapy.
Authors:
E F Bernstein; W S Friauf; P D Smith; J W Cole; R E Solomon; J F Fessler; G F Thomas; C Black; A Russo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of dermatology     Volume:  127     ISSN:  0003-987X     ISO Abbreviation:  Arch Dermatol     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1993-07-29     Completed Date:  1993-07-29     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  0372433     Medline TA:  Arch Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1794-8     Citation Subset:  AIM; IM    
Affiliation:
Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Md.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dihematoporphyrin Ether / analysis*
Female
Fluorometry / instrumentation*,  methods
Guinea Pigs
Photochemotherapy / instrumentation*
Skin / chemistry*
Chemical
Reg. No./Substance:
97067-70-4/Dihematoporphyrin Ether

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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