Document Detail


Transcripts from the cellular homologs of retroviral oncogenes: distribution among chicken tissues.
MedLine Citation:
PMID:  14582157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The oncogenes (v-onc genes) of rapidly transforming retroviruses have homologs (c-onc genes) in the genomes of normal cells. In this study, we characterized and quantitated transcription from four c-onc genes, c-myb, c-myc, c-erb, and c-src, in a variety of chicken cells and tissues. Electrophoretic analysis of polyadenylated RNA, followed by transfer to nitrocellulose and hybridization to cloned onc probes showed that c-myb, c-myc, and c-src each give rise to a single mature transcript, whereas c-erb gives rise to multiple transcripts (B. Vennstrom and J. M. Bishop, Cell, in press) which vary in abundance among different cells and tissues. Transcription from c-myb, c-myc, c-erb, and c-src was quantitated by a "dot-blot" hybridization assay. We found that c-myc, c-erb, and c-src transcription could be detected in nearly all cells and tissues examined, whereas c-myb transcription was detected only in some hemopoietic cells; these cells, however, belong to several different lineages. Thus, in no case was expression of a c-onc gene restricted to a single cell lineage. There appeared to be a correlation between levels of c-myb expression and hemopoietic activity of the tissues and cells examined, which suggests that c-myb may be expressed primarily in immature hemopoietic cells. An examination of c-onc RNA levels in target cells and tissues for viruses carrying the corresponding v-onc genes revealed no obvious correlation, direct or inverse, between susceptibility to transformation by a given v-onc gene and expression of the homologous c-onc gene.
Authors:
T J Gonda; D K Sheiness; J M Bishop
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  2     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1982 Jun 
Date Detail:
Created Date:  2003-10-29     Completed Date:  2003-11-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  617-24     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, University of California, San Francisco, California 94143, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Cell Transformation, Viral / genetics
Cells, Cultured
Chick Embryo
Chickens / genetics*
Gene Expression Profiling*
Hematopoiesis / genetics
Immunoblotting
Oncogene Proteins, Viral / genetics*
Organ Specificity
Proto-Oncogenes / genetics*
RNA, Messenger / genetics,  metabolism
Transcription, Genetic / genetics*
Grant Support
ID/Acronym/Agency:
08T2 CA 09043A/CA/NCI NIH HHS; CA 12705B/CA/NCI NIH HHS; CA 19287B/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Oncogene Proteins, Viral; 0/RNA, Messenger
Comments/Corrections

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