Document Detail

Transcriptomics approach to investigate zebrafish heart regeneration.
MedLine Citation:
PMID:  20179605     Owner:  NLM     Status:  MEDLINE    
In mammals, after a myocardial infarction episode, the damaged myocardium is replaced by scar tissue with negligible cardiomyocyte proliferation. Zebrafish, in contrast, display an extensive regenerative capacity, as they are able to restore completely lost cardiac tissue after partial ventricular amputation. Although questions about the early signals that drive the regenerative response and the relative role of each cardiac cell type in this process still need to be answered, the zebrafish is emerging as a very valuable tool to understand heart regeneration and to devise strategies that may be of potential value to treat human cardiac disease. Here, we performed a genome-wide transcriptome profile analysis focusing on the early time points of zebrafish heart regeneration and compared our results with those of previously published data. Our analyses confirmed the differential expression of several transcripts and identified additional genes whose expression is differentially regulated during zebrafish heart regeneration. We validated the microarray data by conventional and/or quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). For a subset of these genes, their expression pattern was analyzed by in-situ hybridization and shown to be upregulated in the regenerating area of the heart. Our results offer new insights into the biology of heart regeneration in the zebrafish and, together with future experiments in mammals, may be of potential interest for clinical applications.
Eduard Sleep; Stéphanie Boué; Chris Jopling; Marina Raya; Angel Raya; Juan Carlos Izpisua Belmonte
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Journal of cardiovascular medicine (Hagerstown, Md.)     Volume:  11     ISSN:  1558-2035     ISO Abbreviation:  J Cardiovasc Med (Hagerstown)     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-30     Completed Date:  2010-06-17     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  101259752     Medline TA:  J Cardiovasc Med (Hagerstown)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  369-80     Citation Subset:  IM    
Center for Regenerative Medicine, Barcelona, Spain.
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MeSH Terms
Cathepsins / metabolism
Cell Cycle Proteins / metabolism
Gene Expression Profiling*
Heart / physiology*
Myocardium / metabolism*
Oligonucleotide Array Sequence Analysis
Protein-Serine-Threonine Kinases / metabolism
Proto-Oncogene Proteins / metabolism
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Proto-Oncogene Proteins; EC Kinases; EC kinase 1; EC 3.4.-/Cathepsins
Comment In:
J Cardiovasc Med (Hagerstown). 2010 Sep;11(9):631-2   [PMID:  20671525 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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