| Transcriptome of pituitary adenylate cyclase-activating polypeptide-differentiated PC12 cells. | |
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MedLine Citation:
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PMID: 15518888 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Addition of pituitary adenylate cyclase-activating polypeptide (PACAP) into the cultured PC12 cells secreted dopamine and promoted neurite outgrowth of the cells, indicating cell differentiation. To characterize the PACAP-differentiated PC12 cell transcriptome, we applied DNA macroarray techniques, using Atlas Rat 1.2 Array membranes (BD Biosciences Clontech) that have 1176 cDNA. RNA samples were harvested from PC12 cells before and at a time of 6 h treatment with 1 nM PACAP, when neuritogenesis was remarkably observed under the condition used. Several genes regulated by PACAP have been associated with neuritogenesis (i.e. villin 2 and tissue plasminogen activator) or cell growth/differentiation (i.e. cyclin or ornitine decarboxylase). Also, cytoskeleton proteins such as actin or tubulin were up-regulated for cell morphology remodeling. A message of vehicle trafficking molecule (synaptotagmin IV) was more remarkably increased (3.95-6.85-fold). Signaling molecules such as small G proteins (rab12, rab16, or ral), IkappaB, or STAT3 were altered by PACAP. It is noteworthy that PACAP inhibited the expression of galanin receptor 2, whose ligand was shown to inhibit tyrosine hydroxylase activity. Thus, in this study the transcriptome of PACAP-differentiated PC12 was established, leading to the elucidation of the molecular mechanism of neuritogenesis by the neuropeptide. |
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Authors:
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Masami Ishido; Yoshinori Masuo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Regulatory peptides Volume: 123 ISSN: 0167-0115 ISO Abbreviation: Regul. Pept. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-11-02 Completed Date: 2005-03-31 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8100479 Medline TA: Regul Pept Country: Netherlands |
Other Details:
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Languages: eng Pagination: 15-21 Citation Subset: IM |
Affiliation:
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Endocrine Disruptors and Dioxin Research Projects, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan. ishidou@nies.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Differentiation / drug effects*, genetics* DNA, Complementary / genetics Gene Expression / drug effects Gene Expression Profiling Genes, fos Immunohistochemistry Nerve Growth Factors / pharmacology* Neuropeptides / pharmacology* Neurotransmitter Agents / pharmacology* Oligonucleotide Array Sequence Analysis PC12 Cells Pituitary Adenylate Cyclase-Activating Polypeptide Rats Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic / drug effects Tyrosine 3-Monooxygenase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Adcyap1 protein, rat; 0/DNA, Complementary; 0/Nerve Growth Factors; 0/Neuropeptides; 0/Neurotransmitter Agents; 0/Pituitary Adenylate Cyclase-Activating Polypeptide; EC 1.14.16.2/Tyrosine 3-Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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