Document Detail

Transcriptome of pituitary adenylate cyclase-activating polypeptide-differentiated PC12 cells.
MedLine Citation:
PMID:  15518888     Owner:  NLM     Status:  MEDLINE    
Addition of pituitary adenylate cyclase-activating polypeptide (PACAP) into the cultured PC12 cells secreted dopamine and promoted neurite outgrowth of the cells, indicating cell differentiation. To characterize the PACAP-differentiated PC12 cell transcriptome, we applied DNA macroarray techniques, using Atlas Rat 1.2 Array membranes (BD Biosciences Clontech) that have 1176 cDNA. RNA samples were harvested from PC12 cells before and at a time of 6 h treatment with 1 nM PACAP, when neuritogenesis was remarkably observed under the condition used. Several genes regulated by PACAP have been associated with neuritogenesis (i.e. villin 2 and tissue plasminogen activator) or cell growth/differentiation (i.e. cyclin or ornitine decarboxylase). Also, cytoskeleton proteins such as actin or tubulin were up-regulated for cell morphology remodeling. A message of vehicle trafficking molecule (synaptotagmin IV) was more remarkably increased (3.95-6.85-fold). Signaling molecules such as small G proteins (rab12, rab16, or ral), IkappaB, or STAT3 were altered by PACAP. It is noteworthy that PACAP inhibited the expression of galanin receptor 2, whose ligand was shown to inhibit tyrosine hydroxylase activity. Thus, in this study the transcriptome of PACAP-differentiated PC12 was established, leading to the elucidation of the molecular mechanism of neuritogenesis by the neuropeptide.
Masami Ishido; Yoshinori Masuo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Regulatory peptides     Volume:  123     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-02     Completed Date:  2005-03-31     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  15-21     Citation Subset:  IM    
Endocrine Disruptors and Dioxin Research Projects, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
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MeSH Terms
Base Sequence
Cell Differentiation / drug effects*,  genetics*
DNA, Complementary / genetics
Gene Expression / drug effects
Gene Expression Profiling
Genes, fos
Nerve Growth Factors / pharmacology*
Neuropeptides / pharmacology*
Neurotransmitter Agents / pharmacology*
Oligonucleotide Array Sequence Analysis
PC12 Cells
Pituitary Adenylate Cyclase-Activating Polypeptide
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic / drug effects
Tyrosine 3-Monooxygenase / metabolism
Reg. No./Substance:
0/Adcyap1 protein, rat; 0/DNA, Complementary; 0/Nerve Growth Factors; 0/Neuropeptides; 0/Neurotransmitter Agents; 0/Pituitary Adenylate Cyclase-Activating Polypeptide; EC 3-Monooxygenase

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