Document Detail

Transcriptome assembly and isoform expression level estimation from biased RNA-Seq reads.
MedLine Citation:
PMID:  23060617     Owner:  NLM     Status:  MEDLINE    
MOTIVATION: RNA-Seq uses the high-throughput sequencing technology to identify and quantify transcriptome at an unprecedented high resolution and low cost. However, RNA-Seq reads are usually not uniformly distributed and biases in RNA-Seq data post great challenges in many applications including transcriptome assembly and the expression level estimation of genes or isoforms. Much effort has been made in the literature to calibrate the expression level estimation from biased RNA-Seq data, but the effect of biases on transcriptome assembly remains largely unexplored.
RESULTS: Here, we propose a statistical framework for both transcriptome assembly and isoform expression level estimation from biased RNA-Seq data. Using a quasi-multinomial distribution model, our method is able to capture various types of RNA-Seq biases, including positional, sequencing and mappability biases. Our experimental results on simulated and real RNA-Seq datasets exhibit interesting effects of RNA-Seq biases on both transcriptome assembly and isoform expression level estimation. The advantage of our method is clearly shown in the experimental analysis by its high sensitivity and precision in transcriptome assembly and the high concordance of its estimated expression levels with quantitative reverse transcription-polymerase chain reaction data.
AVAILABILITY: CEM is freely available at
SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
Wei Li; Tao Jiang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-10-11
Journal Detail:
Title:  Bioinformatics (Oxford, England)     Volume:  28     ISSN:  1367-4811     ISO Abbreviation:  Bioinformatics     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-12     Completed Date:  2013-07-29     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9808944     Medline TA:  Bioinformatics     Country:  England    
Other Details:
Languages:  eng     Pagination:  2914-21     Citation Subset:  IM    
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MeSH Terms
Gene Expression Profiling / methods*
High-Throughput Nucleotide Sequencing / methods*
Protein Isoforms / genetics
Sequence Analysis, RNA / methods*
Transcription, Genetic
Grant Support
Reg. No./Substance:
0/Protein Isoforms

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