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Transcriptome Analysis of CD133-positive Stem Cells and Prognostic Value of Survivin in Colorectal Cancer.
MedLine Citation:
PMID:  25331798     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background/Aim: CD133 is an important, but not exclusive, biomarker of colorectal cancer (CRC) stem cells. Materials and Methods: In order to identify other CRC stem cell-specific genes, we performed a comparative expression profiling of CD133(+) and CD133(-) cell populations in primary and metastatic tumors from four patients with CRC. CD133(+) and CD133(-) CRC cells were isolated using MagSweeper and used for whole-transcriptome analysis with RNA-Seq. Results: We found that in CD133(+) cells, 17 genes (RNASE2, PRB2, IL4, MGC27382, CLEC4C, SALL3, GIMAP1, ISG15, LOC728875, ZIK1, ICAM2, CCDC7, CDYL2, LRRC2, ZEB1, OSTF1 and CCDC144B) were significantly up-regulated compared to CD133(-) CRC cells. Among them, IL4 has been known as an inducer of survivin implicated in the survival and proliferation of cancer cells. However, the prognostic value of survivin in CRC is controversial. We evaluated survivin expression in formalin-fixed paraffin-embedded tumor samples of 188 patients with CRC by immunohistochemistry. Survivin over-expression was detected in 85 patients (45.2%) and was significantly associated with primary tumor sites (p=0.028), lymph node metastasis (p=0.029) and advanced III/IV CRC stages (AJCC 7; p=0.001). Furthermore, survivin up-regulation correlated with reduced disease-free survival (DFS; p=0.021) and overall survival (OS; p<0.000) and was proved to be an independent prognostic factor for both DFS and OS in multivariate analysis. Conclusion: Our data suggest that CD133(+) CRC stem cells have a distinct expression pattern and that survivin, up-regulated by differentially expressed IL-4, is a candidate biomarker for the prediction of recurrence and survival in CRC.
Authors:
Seung Tae Kim; Insuk Sohn; In-Gu DO; Jiryeon Jang; Seok Hyung Kim; In Ho Jung; Joon Oh Park; Young Suk Park; Amirali Talasaz; Jeeyun Lee; Hee Cheol Kim
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Cancer genomics & proteomics     Volume:  11     ISSN:  1790-6245     ISO Abbreviation:  Cancer Genomics Proteomics     Publication Date:    2014 09-10
Date Detail:
Created Date:  2014-10-21     Completed Date:  -     Revised Date:  2014-10-22    
Medline Journal Info:
Nlm Unique ID:  101188791     Medline TA:  Cancer Genomics Proteomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  259-266     Citation Subset:  -    
Copyright Information:
Copyright© 2014, International Institute of Anticancer Research (Dr. John G. Delinasios), All rights reserved.
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