Document Detail


Transcriptionally defective nucleocapsids of vesicular stomatitis virus from cells treated with indomethacin.
MedLine Citation:
PMID:  3027967     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Indomethacin blocks the biosynthesis of vesicular stomatitis virus (VSV) at the level of primary transcription, RNA replication, and protein synthesis (P. K. Mukherjee and R. W. Simpson (1985), Virology 140, 188-191). Nucleocapsids of infecting virus particles recovered from indomethacin-treated cells were analyzed for in vitro transcriptase activity. Incorporation of [3H]UTP in mixtures containing nucleocapsids from HEp-2 cells pretreated with 10(-3) M indomethacin was inhibited approximately 80% compared to control reactions containing nucleocapsids from untreated infected cells. The level of inhibition of in vitro transcriptase activity of viral nucleocapsids from drug-treated cultures varied according to the cell line used for infection. After indomethacin removal, cells regained their ability to produce enzymatically competent viral-transcribing complexes unless they were subsequently exposed to metabolic inhibitors such as actinomycin D or alpha-amanitin. Enzymatically defective nucleocapsids from indomethacin-treated cells showed enhanced in vitro transcriptase activity in the presence of modulators of prostaglandins and cyclic nucleotides. Electrophoretic analysis of product from in vitro transcriptase reactions revealed that these defective nucleocapsids are unable to synthesize VSV messenger RNA or normal size leader RNA species but only smaller transcripts of undetermined identity.
Authors:
P K Mukherjee; R W Simpson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  156     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-03-02     Completed Date:  1987-03-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  25-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Capsid / genetics*,  isolation & purification
Cell Line
Indomethacin / pharmacology*
Kinetics
RNA, Viral / genetics
Transcription, Genetic / drug effects*
Vesicular stomatitis Indiana virus / drug effects,  genetics*
Viral Core Proteins / genetics*,  isolation & purification
Grant Support
ID/Acronym/Agency:
AI-17262/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Viral; 0/Viral Core Proteins; 53-86-1/Indomethacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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