| Transcriptionally defective nucleocapsids of vesicular stomatitis virus from cells treated with indomethacin. | |
| | |
MedLine Citation:
|
PMID: 3027967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Indomethacin blocks the biosynthesis of vesicular stomatitis virus (VSV) at the level of primary transcription, RNA replication, and protein synthesis (P. K. Mukherjee and R. W. Simpson (1985), Virology 140, 188-191). Nucleocapsids of infecting virus particles recovered from indomethacin-treated cells were analyzed for in vitro transcriptase activity. Incorporation of [3H]UTP in mixtures containing nucleocapsids from HEp-2 cells pretreated with 10(-3) M indomethacin was inhibited approximately 80% compared to control reactions containing nucleocapsids from untreated infected cells. The level of inhibition of in vitro transcriptase activity of viral nucleocapsids from drug-treated cultures varied according to the cell line used for infection. After indomethacin removal, cells regained their ability to produce enzymatically competent viral-transcribing complexes unless they were subsequently exposed to metabolic inhibitors such as actinomycin D or alpha-amanitin. Enzymatically defective nucleocapsids from indomethacin-treated cells showed enhanced in vitro transcriptase activity in the presence of modulators of prostaglandins and cyclic nucleotides. Electrophoretic analysis of product from in vitro transcriptase reactions revealed that these defective nucleocapsids are unable to synthesize VSV messenger RNA or normal size leader RNA species but only smaller transcripts of undetermined identity. |
| | |
Authors:
|
P K Mukherjee; R W Simpson |
Related Documents
:
|
3447387 - Higher yields and increased purity of in vitro grown toxoplasma gondii. 12083837 - Target-cell-derived trna-like primers for reverse transcription support retroviral infe... 2155247 - Analysis of bovine parainfluenza-3 virus replication in bovine embryonic lung cells by ... 4286247 - Assay of variola virus by the fluorescent cell-counting technique. 22920837 - Array-based sensing of metastatic cells and tissues using nanoparticle-fluorescent prot... 22383887 - Cell lineage analysis of the mammalian female germline. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Virology Volume: 156 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 1987 Jan |
Date Detail:
|
Created Date: 1987-03-02 Completed Date: 1987-03-02 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0110674 Medline TA: Virology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 25-31 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Capsid / genetics*, isolation & purification Cell Line Indomethacin / pharmacology* Kinetics RNA, Viral / genetics Transcription, Genetic / drug effects* Vesicular stomatitis Indiana virus / drug effects, genetics* Viral Core Proteins / genetics*, isolation & purification |
| Grant Support | |
ID/Acronym/Agency:
|
AI-17262/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Viral; 0/Viral Core Proteins; 53-86-1/Indomethacin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Complete nucleotide sequence of the matrix protein mRNA and three intergenic junctions of human para...
Next Document: Genome of coxsackievirus B3.