| Transcriptional termination signals for RNA polymerase II in fission yeast. | |
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MedLine Citation:
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PMID: 9218804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transcription 'run-on' (TRO) analysis using permeabilized yeast cells indicates that transcription terminates between 180 and 380 bp downstream of the poly(A) site of the Schizosaccharomyces pombe ura4 gene. Two signals direct RNA polymerase II (pol II) to stop transcription: the previously identified 3' end formation signals located close to the poly(A) site and an additional downstream element (DSE) located at the region of termination. The downstream signal (135 bp) appears to act by pausing the elongating polymerase. TRO analysis indicates that elevated levels of transcribing polymerases accumulate over the DSE and that removal of this signal leads to transcription proceeding beyond the normal termination region. Furthermore, when inserted between two competing polyadenylation signals, this DSE increases the utilization of upstream poly(A) sites in vivo. We show that polymerase pausing over an extended region of template ensures termination of pol II transcription close to the poly(A) site. |
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Authors:
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C E Birse; B A Lee; K Hansen; N J Proudfoot |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The EMBO journal Volume: 16 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 1997 Jun |
Date Detail:
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Created Date: 1997-08-08 Completed Date: 1997-08-08 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3633-43 Citation Subset: IM |
Affiliation:
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Sir William Dunn School of Pathology, Chemical Pathology Unit, University of Oxford, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cytochrome c Group
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genetics Cytochromes c* Gene Expression Regulation, Fungal* Genetic Markers Poly A / metabolism RNA Polymerase II / metabolism* Saccharomyces cerevisiae Proteins* Schizosaccharomyces / enzymology, genetics* Terminator Regions, Genetic* Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/CYC1 protein, S cerevisiae; 0/Cytochrome c Group; 0/Genetic Markers; 0/Saccharomyces cerevisiae Proteins; 24937-83-5/Poly A; 9007-43-6/Cytochromes c; EC 2.7.7.-/RNA Polymerase II |
| Comments/Corrections | |
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