Document Detail


Transcriptional responses to epigallocatechin-3 gallate in HT 29 colon carcinoma spheroids.
MedLine Citation:
PMID:  15265009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Catechins have been reported to possess anti-cancer activity in vitro and in vivo. To identify target genes that may be involved in the anti-tumorigenic effect of catechins, gene expression profiles in adherent human HT 29 colon carcinoma cells, in HT 29 spheroids and in epigallocatechin-3 gallate (EGCG)-treated HT 29 cells have been analysed by high-density oligonucleotide microarrays. Treatment of HT 29 cells with EGCG (2.5-50 microm) resulted in a dose-dependent inhibition of spheroid formation of HT 29 cells. Forty transcripts were induced at least twofold in 3-day-old spheroids relative to normal adherent cells using three replicates. Oncogenes like c-fos and c-jun are significantly up-regulated in spheroids. We identified several signal transduction and proliferation genes which are down-regulated in response to EGCG treatment. Increase in the mRNA expression profile of c-Fos correlated well with protein levels in HT 29 spheroids whereas EGCG did not affect protein formation. In agreement with the DNA chip data, IQGAP2 protein was not increased in spheroids but protein formation was totally blocked in EGCG-treated cells. Interestingly, no change in expression of cytotoxic or apoptotic related genes has been observed in EGCG-treated cells. Our findings suggest that EGCG may exert its anti-cancer activity through modulation of expression of a number of genes that are involved in cell proliferation, cell-cell contacts and cell-matrix interactions.
Authors:
Patricia McLoughlin; Monic Roengvoraphoj; Cornelia Gissel; Jürgen Hescheler; Ulrich Certa; Agapios Sachinidis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes to cells : devoted to molecular & cellular mechanisms     Volume:  9     ISSN:  1356-9597     ISO Abbreviation:  Genes Cells     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-21     Completed Date:  2005-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9607379     Medline TA:  Genes Cells     Country:  England    
Other Details:
Languages:  eng     Pagination:  661-9     Citation Subset:  IM    
Affiliation:
Roche Centre for Medical Genomics, F. Hoffmann-La Roche, Ltd, Postfach, 4070 Basel, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Catechin / analogs & derivatives*,  pharmacology*
Colonic Neoplasms / genetics*,  pathology
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Neoplastic / drug effects
HT29 Cells
Humans
Oligonucleotide Array Sequence Analysis
Proto-Oncogene Proteins c-fos / metabolism
RNA, Messenger / genetics
ras GTPase-Activating Proteins / metabolism
Chemical
Reg. No./Substance:
0/IQGAP2 protein, human; 0/Proto-Oncogene Proteins c-fos; 0/RNA, Messenger; 0/ras GTPase-Activating Proteins; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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