Document Detail


Transcriptional reprogramming during reloading of atrophied rat soleus muscle.
MedLine Citation:
PMID:  15956763     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hypothesis was tested that differential, coregulated transcriptional adaptations of various cellular pathways would occur early with increased mechanical loading of atrophied skeletal muscle and relate to concurrent damage of muscle fibers. Atrophy and slow-to-fast fiber transformation of rat soleus muscle was provoked by 14 days of hindlimb suspension (HS). Subsequent reloading of hindlimbs caused a fourfold increase in the percentage of muscle fibers, demonstrating endomysial tenascin-C staining. Five days after reloading, when 10% of the fibers were damaged, the normal muscle weight and slow-type fiber percentage were reestablished. Microarray analysis revealed major, biphasic patterns of gene expressional alterations with reloading that distinguish between treatments and gene ontologies. Transcript levels of factors involved in protein synthesis and certain proteasomal mRNAs were increased after 1 day of reloading and correlated to the percentage of fibers surrounded by tenascin-C. By contrast, levels of gene messages for fatty acid transporters, respiratory chain constituents, and voltage-gated cation channels were transiently reduced after 1 day of muscle loading and associated with the number of damaged fibers and the regain in muscle weight. This coregulation points toward important retooling of oxidative metabolism and the T- and SR-tubular systems with rebuilding of slow fibers. The observations demonstrate that early nuclear reprogramming with reloading of atrophic soleus muscle is coordinated and links to the processes involved in mechanical damage and regeneration of muscle fibers.
Authors:
Martin Flück; Silvia Schmutz; Matthias Wittwer; Hans Hoppeler; Dominique Desplanches
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  289     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-15     Completed Date:  2005-07-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R4-14     Citation Subset:  IM; S    
Affiliation:
Department of Anatomy, University of Bern, Baltzerstr. 2, 3000 Bern 9, Switzerland. flueck@ana.unibe.ch
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological
Animals
Female
Hindlimb
Hindlimb Suspension
Motor Activity*
Muscle Fibers, Skeletal / pathology
Muscle, Skeletal / metabolism,  pathology,  physiopathology*
Muscular Atrophy / genetics,  metabolism,  pathology,  physiopathology*
Oligonucleotide Array Sequence Analysis
Organ Size
RNA, Messenger / metabolism
Rats
Rats, Wistar
Time Factors
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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