Document Detail


Transcriptional repression of MMP-1 by p21SNFT and reduced in vitro invasiveness of hepatocarcinoma cells.
MedLine Citation:
PMID:  15467742     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
p21SNFT (21 kDa small nuclear factor isolated from T cells) is a human basic leucine zipper transcription factor that can repress AP-1-mediated transcription. We show here that overexpression of p21SNFT in HepG2 cells leads to repression of matrix metalloproteinase-1 by 70-80%. p21SNFT interacted with Jun at the matrix metalloproteinase-1 promoter -88 Ets/AP-1 enhancer element, where Jun is known to activate transcription via interaction with Fos and Ets proteins. When p21SNFT/Jun dimers bound the element in the presence of Ets, DNA was protected differently than when Fos was paired with Jun. The data suggest a difference in overall conformation between p21SNFT-containing and Fos-containing complexes that may be involved in the repression of matrix metalloproteinase-1 by p21SNFT. Overexpression of p21SNFT led to a reduction in invasiveness of HepG2 cells through type I collagen and reconstituted basement membrane, an effect similar to that obtained via direct immunodepletion of matrix metalloproteinase-1. The results indicate that the mechanism of repression of matrix metalloproteinase-1 by p21SNFT may be exploited in inhibiting pathological matrix remodeling during cancer progression in vivo.
Authors:
Kristen E Bower; Jamie M Fritz; Kathleen L McGuire
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  23     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-19     Completed Date:  2005-03-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  8805-14     Citation Subset:  IM    
Affiliation:
Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-4614, USA. kbower@scripps.edu
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Basic-Leucine Zipper Transcription Factors
Carcinoma, Hepatocellular / enzymology,  genetics,  pathology*
Cell Line, Tumor
Collagen / physiology
DNA Primers
Electrophoretic Mobility Shift Assay
Enhancer Elements, Genetic
Humans
Immunoprecipitation
Liver Neoplasms / enzymology,  genetics,  pathology*
Matrix Metalloproteinase 1 / genetics*
Neoplasm Invasiveness / genetics*
Promoter Regions, Genetic
Repressor Proteins / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors / physiology*
Transcription, Genetic / physiology*
Chemical
Reg. No./Substance:
0/Basic-Leucine Zipper Transcription Factors; 0/DNA Primers; 0/Repressor Proteins; 0/SNFT protein, human; 0/Transcription Factors; 9007-34-5/Collagen; EC 3.4.24.7/Matrix Metalloproteinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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